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Flag Tag Monoclonal Antibody

Datasheet
Code CSB-MA000021M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blotting(WB) - Flag Tag Monoclonal Antibody

    Western Immunoblot analysis of randomly picked 7 differend DDK-tagged overexpressio lysates with CSB-MA000021M0m at 1:2000

  • Immunofluorescence(IF) - Flag Tag Monoclonal Antibody

    IF:Hela cells transfected with DDK-tagged CD146 ORF cDNA clone were immunostained with M0002 antibody,and then colored in greed with FITC conjugated Secondary antibody(1:2000).The nuclei(blue) were counterstained with DAPI.

  • Immunoprecipitation(IP) - Flag Tag Monoclonal Antibody

    IP-WB:Result of anti DDK-tag Monoclonal antibody
    Lane 1:DDK-tagged fusion protein
    Transfected HEK-293 cells lysate
    Lane 2:Elution
    Lane 3:Precipitateing DDK-tagged fusion protein
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size : 40kd
    Observed band size : 40kd
Immunogen DYKDDDDK(FLAG) synthetic peptide conjugate to KLH
Raised in mouse
Tested Applications ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000
Relevance FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Form liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%,Protein G purified
Isotype IgG2a
Clonality monoclonal
Alias 3X Flag, DDK tag, DYKDDDDK, Flag, Flag Tag
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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