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Your Good Partner in Biology Research
| Code | CSB-MA000021M0m |
| Product Type | Monoclonal Antibody |
| Size |
US$200Purchase it in Cusabio online store (Shop online in the US. Customers outside the US, please order from local distributors.) |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunoprecipitation (IP) Protocol |
| Immunogen | DYKDDDDK(Flag) synthetic peptide conjugate to KLH |
| Raised in | Mouse |
| Tested Applications | ELISA, WB, IP; Recommended dilution: WB:1:1000-1:10000, IP: 0.5µl-2µl |
| Relevance | Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. |
| Form | Liquid |
| Conjugate | Non-conjugated |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method | >95%, Protein G purified |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Alias | DDK tag, DYKDDDDK, Flag, Flag Tag |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Clone No. | 4G5C8 |
| Still Have Questions? | Leave a Message or Start an on-line Chat |
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