Flag Tag Monoclonal Antibody

Datasheet
Code CSB-MA000021M0m
Product Type Monoclonal Antibody
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  • Western Blot
    Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 59.6, 36, 35, 42 kDa
    Observed band size: 59.6, 36, 35, 42 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 10ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: 5 different recombinant proteins with Flag Tag
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
     

  • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
    Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
    Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
    For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
     

  • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)
    Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Immunogen DYKDDDDK(Flag) synthetic peptide conjugate to KLH
Raised in Mouse
Tested Applications ELISA, WB, IP; Recommended dilution: WB:1:1000-1:10000, IP: 0.5µl-2µl
Relevance Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG2a
Clonality Monoclonal
Alias DDK tag, DYKDDDDK, Flag, Flag Tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 4G5C8
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