Flag Tag Monoclonal Antibody

Code CSB-MA000021M0m
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
    All lanes: Flag Tag antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 59.6, 36, 35, 42 kDa
    Observed band size: 59.6, 36, 35, 42 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 10ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa
     

  • Western Blot
    Positive WB detected in: 5 different recombinant proteins with Flag Tag
    All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/50000 dilution
     

  • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
    Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
    Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
    For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
     

  • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)
    Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)

  • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.

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Product Details

Target Names Flag Tag
Alternative Names DDK tag, DYKDDDDK, Flag, Flag Tag
Raised in Mouse
Species Reactivity N/A
Immunogen DYKDDDDK(Flag) synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2a
Clone No. 4G5C8
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:10000
IF 1:100-1:300
IP 0.5μl-2μl
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication. Badham M, et al,University of Kent,2018

Sample type: HEK-293T cells
Sample species: Human
Application: MASS SPECTROSCOPY
Review: Cells were lysed and immunoprecipitated using monoclonal IgG2b anti-Flag antibody.

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