Flag-Tag Monoclonal Antibody

The high hydrophilicity and compact nature of the FLAG tag (DYKDDDDK) allow it to be seamlessly fused to either the N- or C-terminus of target proteins with minimal disruption to their native structure and function. This widespread adoption stems from its exceptional utility in affinity purification, enzymatic cleavage, and sensitive immunological detection across diverse expression systems.

The Mouse Anti-FLAG-Tag Monoclonal Antibody (CSB-MA249923, IgG1 isotype) exemplifies high-quality antibody production. Engineered through rigorous hybridoma technology and subsequently refined via affinity chromatography, this reagent ensures exceptional monospecificity and lot-to-lot reproducibility.

Validated for Enzyme-Linked Immunosorbent Assay (ELISA) and Flow Cytometry (FC), this antibody demonstrates outstanding reactivity in live-cell analyses. As depicted in the flow cytometry overlay histogram, the reagent successfully identifies transiently transfected 293 cells harboring the FLAG-tagged construct. In this stringent side-by-side comparison, the untransfected parental control cells (green peak) exhibit negligible background fluorescence. Conversely, the FLAG-transfected cohort (magenta peak) displays a dramatic rightward shift in fluorescence intensity, unequivocally confirming the antibody's capacity to detect low-abundance epitopes with high fidelity.

This monoclonal antibody is an indispensable tool for researchers interrogating protein expression dynamics, validating recombinant constructs, or isolating native protein complexes via immunoprecipitation. Its proven performance in flow cytometry underscores its utility in high-throughput screening and cell sorting applications, solidifying its role as a reliable workhorse reagent in cell biology and biotechnology.