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GFP monoclonal antibody CSB-MA000051M0m was produced in the mouse immunized by using the Recombinant GFP Protein as the immunogen. The target protein GFP is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
This GFP Monoclonal Antibody was tested in the ELISA, WB and IP. The non-conjugated IgG2b got purified by protein G and reached up to 95% in purity. Anti-GFP antibodies provide a convenient method for visualizing GFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. It doesn’t have species restricted.
CFP, eGFP, eYFP, GFP, GFP tag, YFP
|Immunogen||Recombinant GFP Protein|
|Purification Method||>95%,Protein G purified|
|Concentration||It differs from different batches. Please contact us to confirm it.|
|Buffer||Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4|
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
|Troubleshooting and FAQs||Antibody FAQs|
|Storage||Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.|
|Lead Time||Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
Applications : IHC
Sample dilution: 1: 500
Review: fluorescent microscopic analysis 12 h after inoculation showing the fungal hyphae expressing GFP (in red) penetrating the floral tissue (in blue).
Applications : IP
Sample dilution: 1: 1000
Review: HEK293T cells were transfected with Flag-PSMA8 and GFP-SYCP1. Protein complexes were immunoprecipitated overnight with either an anti-Flag or anti-EGFP or IgGs (negative control), and were analyzed by immunoblotting with the indicated antibody. PSMA8 co-immunoprecipitates with SYCP1.
Applications : IP
Review: PCV2 Cap interacts with pDNAJB6 in transfected cells. HEK293T cells were co-transfected with GFP-Cap and Flag-pDNAJB6 expression plasmids or co-transfected with the pEGFP-N1 control vector and the Flag-pDNAJB6 expression plasmid. The cells transfected with pEGFP-N1 control vector, GFP-Cap expression plasmid or Flag-pDNAJB6 expression plasmid alone served as controls. The interaction of GFP-Cap with Flag-pDNAJB6 was identified through immunoprecipitation using anti-Flag antibodies or anti-GFP antibodies.