GFP Monoclonal Antibody

Datasheet
Code CSB-MA000051M0m
See More Details Free Antibody trial simple
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • WB: Mouse Anti-GFP monoclonal antibody at 0.1μg/ml
    Line 1:HEK-293 cell lysate
    Line 2:GFP transfected HEK-293 cell lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size : 30kd
    Observed band size : 30kd

  •  

    IP:Result of anti-GFP-tag Monoclonal antibody
    Line 1:Control IgG
    Line 2:Precipitateing GFP-tagged fusion protein
    Line 3:GFP Transfected HEK-293 cells lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size : 30kd
    Observed band size : 30kd

Product Details

Target Names GFP
Alternative Names CFP, eGFP, eYFP, GFP, GFP tag, YFP
Raised in mouse
Species Reactivity N/A
Immunogen Recombinant GFP Protein
Conjugate Non-conjugated
Clonality monoclonal
Isotype IgG2b
Purification Method >95%,Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form liquid
Tested Applications ELISA,WB,IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. Gomez-H L, et al,Plos Genetics,2019

Applications: IP
Antibody dilution factor: 1: 1000
Review: HEK293T cells were transfected with Flag-PSMA8 and GFP-SYCP1. Protein complexes were immunoprecipitated overnight with either an anti-Flag or anti-EGFP or IgGs (negative control), and were analyzed by immunoblotting with the indicated antibody. PSMA8 co-immunoprecipitates with SYCP1.

Microscopic analysis of colonization of Colletotrichum abscissum in citrus tissues. Daiani CristinaSavi,microbiological research,2019

Applications: IHC
Antibody dilution factor: 1: 500
Review: fluorescent microscopic analysis 12 h after inoculation showing the fungal hyphae expressing GFP (in red) penetrating the floral tissue (in blue).
PMID: 31284941

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