GFP Monoclonal Antibody

Datasheet
Code CSB-MA000051M0m
Product Type Monoclonal Antibody
Size US$200Purchase it in Cusabio online store
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Image
  • WB: Mouse Anti-GFP monoclonal antibody at 0.1μg/ml
    Line 1:HEK-293 cell lysate
    Line 2:GFP transfected HEK-293 cell lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size : 30kd
    Observed band size : 30kd

  •  

    IP:Result of anti-GFP-tag Monoclonal antibody
    Line 1:Control IgG
    Line 2:Precipitateing GFP-tagged fusion protein
    Line 3:GFP Transfected HEK-293 cells lysate
    Secondary
    Goat polyclonal to Mouse IgG at 1/5000 dilution
    Predicted band size : 30kd
    Observed band size : 30kd

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Immunogen Recombinant GFP Protein
Raised in mouse
Tested Applications ELISA,WB,IP;Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000
Relevance The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
Form liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%,Protein G purified
Isotype IgG2b
Clonality monoclonal
Alias CFP, eGFP, eYFP, GFP, GFP tag, YFP
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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