Strep-Tag Monoclonal Antibody

The Strep-Tag, a short peptide sequence derived from the biotin-binding domain of streptavidin, serves as a powerful affinity handle for recombinant protein purification and detection. By leveraging the high-specificity interaction with Strep-Tactin® matrices, this tag enables efficient, one-step isolation of target proteins under gentle, physiological conditions, thereby preserving their native conformation and biological activity.

The Mouse Anti-Strep-Tag Monoclonal Antibody (CSB-MA000171, IgG isotype) delivers exceptional specificity and binding affinity for this epitope. Produced via rigorous hybridoma technology utilizing a synthetic peptide immunogen, this reagent ensures lot-to-lot consistency and reliable performance across diverse experimental platforms.

Validated for both ELISA and Western Blot applications, this antibody exhibits outstanding sensitivity. As demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 1 µg of Strep II fusion protein at high working dilutions of 1:5,000 and 1:10,000. The presence of crisp, intensely staining bands at both concentrations—free from background interference or non-specific binding—confirms the antibody’s superior immunoreactivity and high signal-to-noise ratio. Its robust performance at low concentrations makes it an economical and powerful tool for high-throughput screening and detailed molecular characterization.

This monoclonal antibody is an indispensable asset for researchers engaged in protein engineering, structural biology, and cell signaling studies. It provides a trusted means to verify Strep-tagged protein expression, assess molecular weight, and monitor purification efficiency. With its proven reliability and versatility, the Strep-Tag Monoclonal Antibody consistently delivers high-fidelity results, empowering breakthrough discoveries in modern life sciences.