301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
|Source||in vitro E.coli expression system|
|Have Questions?||Leave a Message or Start an on-line Chat|
W02F12.2; Alkaline ceramidase; AlkCDase; Alkaline N-acylsphingosine amidohydrolase; Alkaline acylsphingosine deacylase
|Target Protein Sequence||MESSSINRWFEYESGHAWCESAYKYQTLPYVAEFANTCTNLPIIVLPLVNIMLLRRYLHD VNGGLIFPQLLLTFNGLASTYYHATLNLFGQLVDELSLVWIITVFLVVYIPVMKWFPEKF SKRLTLVRWVVLIVTALVSGLCFLEPNLNAIALMLFSIPAAVVINYEGKQSGIPDIESFP SRILALWGVAFSFWFADRLLCDFWLYLGTPYLHALFHLLAGLAGYTIFIMFSMIDIESRT KTHKYTAAVRYFPGKNGSIFSFPYISLKERSQ|
|Protein Length||full length protein|
|Tag Info|| The following tags are available.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer before Lyophilization||Tris/PBS-based buffer, 6% Trehalose, pH 8.0|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||Delivery time
may differ from different purchasing way or location, please kindly consult your local distributors
for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet||Please contact us to get it.|
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Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
|Subcellular Location||Membrane; Multi-pass membrane protein.|
|Protein Families||Alkaline ceramidase family|