Recombinant Arabidopsis thaliana 3-ketoacyl-CoA synthase 17 (KCS17)

Code CSB-CF713572DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
KCS2
Uniprot No.
Alternative Names
KCS2; DAISY; KCS17; At1g04220; F20D22.1; 3-ketoacyl-CoA synthase 2; KCS-2; Docosanoic acid synthase; Very long-chain fatty acid condensing enzyme 2; VLCFA condensing enzyme 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-528
Target Protein Sequence
MNENHIQSDHMNNTIHVTNKKLPNFLLSVRLKYVKLGYHYLISNAVYILILPVGLLAATS SSFSLTDLTLLYNHLLKFHFLSSTLFAALLIFLTTLYFTTRPRRIFLLDFACYKPDSSLI CTRETFMDRSQRVGIFTEDNLAFQQKILERSGLGQKTYFPEALLRVPPNPCMSEARKEAE TVMFGAIDAVLEKTGVNPKDIGILVVNCSLFNPTPSLSAMIVNKYKLRGNVLSYNLGGMG CSAGLISIDLAKQLLQVQPNSYALVVSTENITLNWYLGNDRSMLLSNCIFRMGGAAVLLS NRSSDRCRSKYQLIHTVRTHKGSDDNAFNCVYQREDNDDNKQIGVSLSKNLMAIAGEALK TNITTLGPLVLPMSEQLLFFATLVARKVFNVKKIKPYIPDFKLAFEHFCIHAGGRAVLDE IEKNLDLSEWHMEPSRMTLNRFGNTSSSSLWYELAYSEAKGRIKRGDRTWQIAFGSGFKC NSAVWRALRTIDPSKEKKKKTNPWIDEIHEFPVPVPRTSPVTSSSESR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates the synthesis of VLCFAs from 22 to 26 carbons in length (e.g. C22, C24, C26). Involved in the elongation of C20 fatty acid suberin precursors. Functionally redundant with KCS20 in the two-carbon elongation of C22 fatty acids that is required for cuticular wax and root suberin biosynthesis.
Gene References into Functions
  1. DAISY is transcriptionally activated by NaCl application, and also by polyethylene glycol-induced drought stress and wounding, and is involved in suberin biosynthesis. PMID: 18786002
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Chalcone/stilbene synthases family
Tissue Specificity
Expressed in siliques, flowers and stems. In young seedlings, expressed in the central cylinder of primary roots, in emerging lateral roots and in their root cap, but not in aboveground tissues such as hypocotyls, cotyledons and leaves. Expressed in sepal
Database Links

KEGG: ath:AT1G04220

UniGene: At.20865

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