Recombinant Arabidopsis thaliana 3-ketoacyl-CoA synthase 6 (CUT1)

Code CSB-CF895425DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
CUT1
Uniprot No.
Alternative Names
CUT1; CER6; EL6; KCS6; At1g68530; T26J14.10; 3-ketoacyl-CoA synthase 6; KCS-6; Cuticular protein 1; Very long-chain fatty acid condensing enzyme 6; VLCFA condensing enzyme 6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-497
Target Protein Sequence
MPQAPMPEFSSSVKLKYVKLGYQYLVNHFLSFLLIPIMAIVAVELLRMGPEEILNVWNSL QFDLVQVLCSSFFVIFISTVYFMSKPRTIYLVDYSCYKPPVTCRVPFATFMEHSRLILKD KPKSVEFQMRILERSGLGEETCLPPAIHYIPPTPTMDAARSEAQMVIFEAMDDLFKKTGL KPKDVDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGLISVDLARDLLQ VHPNSNAIIVSTEIITPNYYQGNERAMLLPNCLFRMGAAAIHMSNRRSDRWRAKYKLSHL VRTHRGADDKSFYCVYEQEDKEGHVGINLSKDLMAIAGEALKANITTIGPLVLPASEQLL FLTSLIGRKIFNPKWKPYIPDFKLAFEHFCIHAGGRAVIDELQKNLQLSGEHVEASRMTL HRFGNTSSSSLWYELSYIESKGRMRRGDRVWQIAFGSGFKCNSAVWKCNRTIKTPKDGPW SDCIDRYPVFIPEVVKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Required for elongation of C24 fatty acids, an essential step of the cuticular wax production. Major condensing enzyme for stem wax and pollen coat lipid biosynthesis.
Gene References into Functions
  1. CUT1 is providing wax signals for germination of powdery mildew fungi on Arabidopsis. PMID: 25201879
  2. Results show that alcohol levels at the site of biosynthesis are mainly limiting the ester formation in the Arabidopsis CER6 wild-type epidermis. PMID: 17376164
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Chalcone/stilbene synthases family
Tissue Specificity
In epidermal cells of aerial tissues and in the tapetum of anthers near maturity. Expressed in siliques, flowers and leaves.
Database Links

KEGG: ath:AT1G68530

STRING: 3702.AT1G68530.1

UniGene: At.23200

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