Recombinant Arabidopsis thaliana ATP-dependent zinc metalloprotease FTSH 1, chloroplastic (FTSH1)

Code CSB-CF655100DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
FTSH1
Uniprot No.
Alternative Names
FTSH1; AAA; FTSH; At1g50250; F14I3.14; ATP-dependent zinc metalloprotease FTSH 1, chloroplastic; AtFTSH1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
87-716
Target Protein Sequence
VVDEPASPSVVIESQAVKPSTPSPLFIQNEILKAPSPKSSDLPEGSQWRYSEFLNAVKKG KVERVRFSKDGSVVQLTAVDNRRASVIVPNDPDLIDILAMNGVDISVSEGESSGNDLFTV IGNLIFPLLAFGGLFLLFRRAQGGPGGGPGGLGGPMDFGRSKSKFQEVPETGVSFADVAG ADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGPPGTGKTLLARAVAGEAGVPFFSC AASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEIDAVGRQRGAGMGGGNDEREQTIN QLLTEMDGFSGNSGVIVLAATNRPDVLDSALLRPGRFDRQVTVDRPDVAGRVKILQVHSR GKALGKDVDFDKVARRTPGFTGADLQNLMNEAAILAARRELKEISKDEISDALERIIAGP EKKNAVVSEEKKRLVAYHEAGHALVGALMPEYDPVAKISIIPRGQAGGLTFFAPSEERLE SGLYSRSYLENQMAVALGGRVAEEVIFGDENVTTGASNDFMQVSRVARQMIERFGFSKKI GQVAVGGPGGNPFMGQQMSSQKDYSMATADIVDAEVRELVEKAYKRATEIITTHIDILHK LAQLLIEKETVDGEEFMSLFIDGQAELYIS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions.
Gene References into Functions
  1. Results elucidate protein activity and its amount as important factors for the function of FtsH heterocomplexes that are composed of multiple isoforms in the thylakoid membrane. PMID: 21062893
  2. part of the FtsH hexamers are juxtapositioned to PSII complexes in the grana in darkness, carrying out immediate degradation of the photodamaged D1 protein under light stress PMID: 20921219
Subcellular Location
Plastid, chloroplast thylakoid membrane; Single-pass membrane protein; Stromal side.
Protein Families
AAA ATPase family; Peptidase M41 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT1G50250

STRING: 3702.AT1G50250.1

UniGene: At.21777

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