Recombinant Arabidopsis thaliana Abscisic acid 8'-hydroxylase 2 (CYP707A2)

Code CSB-CF530522DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
CYP707A2
Uniprot No.
Alternative Names
CYP707A2; At2g29090; T9I4.17; Abscisic acid 8'-hydroxylase 2; ABA 8'-hydroxylase 2; Cytochrome P450 707A2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-482
Target Protein Sequence
MQISSSSSSNFFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIG ETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPP SKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQKSIN TLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKAR IELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTA SVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAA SVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYT YMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVT PI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Involved in the control of seed dormancy and germination.
Gene References into Functions
  1. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. PMID: 27731416
  2. Glucose-induced delay in seed germination and seedling development is directly related to the suppression of ABA catabolism through the repression of the CYP707A2 expression. PMID: 21883251
  3. CYP707A2 transcript levels increase from late-maturation to mature dry seed and plays a distinct role in seed development and postgermination growth. PMID: 16543410
  4. central role of the CYP707A2 gene in the nitrate-mediated control of Abscisic acid levels during seed development and germination of arabidopsis thaliana. PMID: 19074630
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Mainly expressed in dry seeds. Lower expression in rosette leaves, flowers, siliques and stems. Not expressed in roots. Expressed in both endosperm and vascular tissues of embryo during the seed development and in cortex and endodermis in germinating embr
Database Links

KEGG: ath:AT2G29090

STRING: 3702.AT2G29090.1

UniGene: At.50108

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