Recombinant Arabidopsis thaliana Copper transporter 1 (COPT1)

Code CSB-CF654393DOA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
COPT1
Uniprot No.
Alternative Names
COPT1; At5g59030; K18B18.10; K18B18.2; Copper transporter 1; AtCOPT1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-170
Target Protein Sequence
MDHDHMHGMPRPSSSSSSSPSSMMNNGSMNEGGGHHHMKMMMHMTFFWGKNTEVLFSGWP GTSSGMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSANRAAGLIQTAVYTLRIGLAYLV MLAVMSFNAGVFLVALAGHAVGFMLFGSQTFRNTSDDRKTNYVPPSGCAC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Copper transporter involved in copper acquisition and transport in leaves. Required for copper homeostasis and normal plant growth and development.
Gene References into Functions
  1. Arabidopsis COPT1 overexpression (C1 (OE) ) in rice causes root shortening in high copper conditions and under iron deficiency. PMID: 28631167
  2. Col-0, high-affinity copper transporter COPT1-overexpressing (C1(OE)) seedlings and T-DNA COPT1 insertion mutant (copt1) differ in their copper-transport activity. C1(OE) showed a fivefold higher Cu-induced K(+) efflux at the root tip compared with Col-0. PMID: 23046313
  3. overexpressing transgenic plants exhibit increased endogenous copper levels and are sensitive to the copper in the growth medium PMID: 20335405
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Copper transporter (Ctr) (TC 1.A.56) family, SLC31A subfamily
Tissue Specificity
Expressed in the root apex, lateral root primordia, embryo, trichomes, guard cells and pollen grains.
Database Links

KEGG: ath:AT5G59030

STRING: 3702.AT5G59030.1

UniGene: At.9700

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