Recombinant Arabidopsis thaliana Mechanosensitive ion channel protein 2, chloroplastic (MSL2)

Code CSB-CF704101DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
MSL2
Uniprot No.
Alternative Names
MSL2; At5g10490; F12B17.160; Mechanosensitive ion channel protein 2, chloroplastic; Mechanosensitive channel of small conductance-like 2; MscS-Like protein 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
76-673
Target Protein Sequence
CHSFSASGKAIEPAVKAVTVVLTKSHGLMQQFPFVYKLVPAVALLVFSLWGLVPFARQGR NILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVLPTEASKIVKDRLLNF VRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKALYSAVWVAAVSLFME LLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVLNEWIQTKIEGYEVSG TVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINN IVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAIL LDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYGGVTSRRPLMLIEPAYKING EDKSKSQNRAAKPTAEQENKGSNPKSKETSSPDLKANVKVGESPVSDTNKVPEETVAKPV IKAVSKPPTPKDTETSGTEKPKAKRSGGTIKSTKTDETDSSTSSASRSTLEENIVLGVAL EGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQKDSQSQPNSGASTEP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Controls plastid size, shape, and perhaps division during normal plant development by altering ion flux in response to changes in membrane tension. Acts as a component of the chloroplast division machinery.
Gene References into Functions
  1. Two of the three conserved motifs are critical for MSL2 function, consistent with the conservation of structure and function between MscS family members in bacteria and plants. PMID: 22768278
  2. MSL2, MSL3, and components of the Min system function in the same pathway to regulate chloroplast size and FtsZ ring formation. PMID: 21810996
  3. We conclude that plastids are under hypoosmotic stress during normal plant growth and dynamic response to this stress requires MSL2 and MSL3. PMID: 22326022
  4. Two MscS-like proteins, MSL2 and MSL3, alter ion flux in response to changes in membrane tension to regulate plastid size, shape, and perhaps division during normal plant development. [MSL2] PMID: 16401419
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein.
Protein Families
MscS (TC 1.A.23) family
Tissue Specificity
Widely expressed.
Database Links

KEGG: ath:AT5G10490

STRING: 3702.AT5G10490.1

UniGene: At.48016

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