Recombinant Arabidopsis thaliana Outer envelope protein 64, chloroplastic (OEP64)

Code CSB-CF862907DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
OEP64
Uniprot No.
Alternative Names
OEP64; TOC64-III; At3g17970; MEB5.19; Outer envelope protein 64, chloroplastic; Translocon at the outer membrane of chloroplasts 64-III
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-589
Target Protein Sequence
MASQAANLWVLLGLGLAGILMLTKKLKKTVREDFGAFIDKLMLLPPPQPAPPKAPHPLTG LTFAVSDVFDITGYVTGFGHPDWVRTHEAASSTSPVVSTLVEGGATCVGKTVVDEFAFSI SGENKHYDSPTNPAAPTRIPGGACSGAAVAVATNAVDFALGIDTVGGVRVPAGYCGVLGF KSSYGAISNTGIIPVSSSLDSVGWFARDPNTLRRVGHVLLQLPFATQRNPRQIILADDCF QLLKIPVDRITQVVTKSAEKLFGRQLLKHQNLETYFETKVPSLKEFARTKAIANTKVSTS RLLANVMQLLQRHEFLQNHGDWINTVKPAIDPVILSQVCENPELTNEETENLNAIRNETR VAIGSLLKDDGILVIPTLPAVPPKLGSKEITSEDYQNRASSLLSIASISGCCQVTVPLGH HEKCPISVSFIGRHGGDRFLLDTVQTMYPSLQEYSSIVTDPKSSKKAITKEESAEIAKEK GNQAFKEKLWQKAIGLYSEAIKLSDNNATYYSNRAAAYLELGGFLQAEEDCTKAITLDKK NVKAYLRRGTAREMLGDCKGAIEDFRYALVLEPNNKRASLSAERLRKFQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Chaperone receptor mediating Hsp90-dependent protein targeting to chloroplasts. Bi-functional preprotein receptor acting on both sides of the membrane. Not essential for an efficient import of pre-proteins into plastids.
Gene References into Functions
  1. Our findings suggest that the TPR domain from AtToc64 has higher affinity towards C-terminal residues of Hsp70. PMID: 24700626
  2. mode of recognition of the C-termini of Hsp70 and Hsp90 chaperones by the Tetratricopeptide Repeat (TPR) domain of the chloroplast import receptor Toc64 PMID: 24391770
  3. Toc64 participates in chloroplast protein import and cooperates in part with Toc33. PMID: 23131143
  4. Toc64 is involved in preprotein translocation across the outer envelope at both sites of the membrane. PMID: 17306301
  5. Data built homology models of the 3-TPR domain of chloroplastic Toc64 from different species. PMID: 19198901

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Subcellular Location
Plastid, chloroplast outer membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed in roots, cotyledons, leaves and flower buds.
Database Links

KEGG: ath:AT3G17970

STRING: 3702.AT3G17970.1

UniGene: At.8043

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