Recombinant Arabidopsis thaliana Probable calcium-activated outward-rectifying potassium channel 6 (KCO6)

Code CSB-CF879862DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
TPK3
Uniprot No.
Alternative Names
TPK3; KCO6; At4g18160; F15J5.130; Two-pore potassium channel 3; AtTPK3; Calcium-activated outward-rectifying potassium channel 6; AtKCO6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-436
Target Protein Sequence
MANEGSDPLLQYMISPRLKKPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRD SSHFIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQPEPWLSDQTSSHCQGHALHR SKTAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQT HPVVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQE SYMLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGW LDSFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRER AKKVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCS NGKITLLDLLEGGSGD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Two-pore potassium channel modulating the proton motive force (pmf) necessary to convert photochemical energy into physiological functions. Mediates the potassium efflux from the thylakoid lumen required for the regulation of the transmembrane electrical potential, the enhancement of the pH gradient for ATP synthesis, the regulation of electron flow, and pH-mediated photoprotective responses. Requires calcium for channel activity.
Gene References into Functions
  1. KCO6 does not form functional potassium channels when expressed in E. coli PMID: 23656881
  2. the TPK3 channel manages the proton motive force necessary to convert photochemical energy into physiological functions. PMID: 24009357
Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein. Note=PubMed:24009357 shows a thylakoid location instead of the tonoplast location previously described.
Protein Families
Two pore domain potassium channel (TC 1.A.1.7) family
Tissue Specificity
Expressed in roots, stems, hypocotyls, leaves and flowers. Detected in root tips and in mesophyll cells and guard cells of the leaves.
Database Links

KEGG: ath:AT4G18160

STRING: 3702.AT4G18160.1

UniGene: At.32938

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