Recombinant Arabidopsis thaliana Probable steroid reductase DET2 (DET2)

Code CSB-CF658520DOA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
DET2
Uniprot No.
Alternative Names
DET2; CRO1; At2g38050; T8P21.4; Steroid 5-alpha-reductase DET2; AtDET2; Protein COMPACT ROSETTE 1; Protein DEETIOLATED 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-262
Target Protein Sequence
MEEIADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPT LWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPITIAAL AFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKE NRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHK WYIAKFKEEYPKTRKAVIPFVY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate.
Gene References into Functions
  1. together with eto1, cesa6(ret8) and det2(ret41) exhibited an enhanced phenotype in the hypocotyls and apical hooks of etiolated seedlings. PMID: 23759102
  2. Down-regulation of the DET2:DWF4:SMT2 genes in transgenic Arabidopsis leads to a selective modification of the campesterol and brassinosteroid profiles. PMID: 20169349
  3. The isolation and characterization of transient defective exine 1 (tde1), an alleles of DET2, is reported. PMID: 18045813
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Steroid 5-alpha reductase family
Database Links

KEGG: ath:AT2G38050

STRING: 3702.AT2G38050.1

UniGene: At.22739

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