Recombinant Arabidopsis thaliana Protein POLLEN DEFECTIVE IN GUIDANCE 1(POD1)

Code CSB-CF518790DOA
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names POD1
Uniprot No. F4HVJ3
Alternative Names POD1; At1g67960; T23K23.19; Protein POLLEN DEFECTIVE IN GUIDANCE 1
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 1-624
Target Protein Sequence MAIRSSGRKLSFEILSQNSSFENDDTSIRRSSSDPITGNVASESPRDYGKRKRSKKKKKK VNQVETILENGDSHSTIITGSSGDFGETTTMFENRLNYYGGGGSGSSGGGCVVTLLDGQT VHHNGFNFGELRQRNVNGSVDGSNDERWSDTLSSDKKLYMEETSVELSPSENPPFQEVQH QFPRSEINGNVVRRLDTEASLDWKQLVADDPDFLSAETRSPMKYFMEEIYGGISLRSTTT PGNDIERERIYDTIFRLPWRCEVLIDTGFFVCVNSFLSLLTVMPIRVLLIFMDAFKNRQF RRPSASELSDLACFLVLATGTILLGRTDISLIYHMIRGQSTIKLYVVYNILEIFDRLCQS FCGDVFGALFSSAKGLSISPPEKLRFSTWRFVSDLALTMAASILHSFILLAQAITLSTCI VAHNNALLALLVSNNFAEIKSSVFKRFSKDNIHGLVYADSIERFHISAFLVSVLAQNILE SEGAWFGNFIYNATTVFFCEMMIDIIKHSFLAKFNDIKPIAYSEFLQALCEQTLNIRPED RKTNLTFVPLAPACVVIRVLTPVYAAHLPYSPLPWRMLWMVILFVITYIMLTSLKVLIGM GLRKHATWYINRCRRRNSSHLHND
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Probable component of the calreticulin 3 (CRT3) complex, acting probably as a co-chaperone involved in protein retention in the endoplasmic reticulum lumen. Required for micropylar pollen tube guidance. Plays an essential role in cell plate orientation or positioning in early embryo patterning.
Gene References into Functions
  1. POD1 is a novel ER luminal protein involved in ER protein retention and interacts with CALRETICULIN3. It plays a specific role in the micropylar response and is also essential for cell patterning during early embryogenesis. PMID: 21954464
Subcellular Location Membrane; Multi-pass membrane protein. Endoplasmic reticulum lumen. Note=Exclusive ER lumen localization and no membrane localization in PubMed:21954464.
Protein Families TAPT1 family
Tissue Specificity Expressed in inflorescences, siliques, roots and shoots. Expressed in early embryo, endosperm, mature pollen and pollen tubes, synergide cells and weakly in antipodal cells.
Database Links

KEGG: ath:AT1G67960

STRING: 3702.AT1G67960.1

UniGene: At.27107

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