Recombinant Arabidopsis thaliana Protein TIC 21, chloroplastic (TIC21)

Code CSB-CF882859DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
TIC21
Uniprot No.
Alternative Names
TIC21; CIA5; PIC1; At2g15290; F27O10.6; Protein TIC 21, chloroplastic; Protein CHLOROPLAST IMPORT APPARATUS 5; AtCIA5; Protein PERMEASE IN CHLOROPLASTS 1; AtPIC1; Translocon at the inner envelope membrane of chloroplasts 21; AtTIC21
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
91-296
Target Protein Sequence
SVPGDNEVDKAKLAQVAKRLEKTSRYFKRLGSIGFWGQLVSTVVAAVILSFSIVVTGKPT SPATFYATASGIAAAFVSVFWSFGYIRLSERLRRTSIDPAKAPPRADVVKGLRSGIMVNI LGMGSALLGMQATVGFLVAKALTTSANPFYQGVSQGYSPVLALDVFLVQASANTLLSHFL GLVCSLELLRSVTVPNSESVVVPKVA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in chloroplast protein import across the inner envelope membrane. Acts also as a chloroplast permease regulating the iron transport and homeostasis. Involved in the uptake and sequestration of iron in plastids.
Gene References into Functions
  1. By mediating iron uptake and sequestering processes in plastids, PIC1 is crucial for plant growth and development in general and in particular for fruit maturation. PMID: 21343424
  2. Tic21 functions as part of the inner membrane protein-conducting channel and may be more important for later stages of leaf development. [Tic21] [CIA5] PMID: 16891400
  3. PIC1 functions in iron transport across the inner envelope of chloroplasts and hence in cellular metal homeostasis. [PIC1] [PERMEASE INCHLOROPLASTS1] PMID: 17337631
Subcellular Location
Plastid, chloroplast inner membrane; Multi-pass membrane protein.
Tissue Specificity
Ubiquitous. Highest expression in green tissues and very low levels in mature pollen.
Database Links

KEGG: ath:AT2G15290

STRING: 3702.AT2G15290.1

UniGene: At.22376

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