Recombinant Arabidopsis thaliana UDP-glucuronate:xylan alpha-glucuronosyltransferase 1 (GUX1)

Code CSB-CF864828DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
GUX1
Uniprot No.
Alternative Names
GUX1; PGSIP1; At3g18660; MVE11.2; UDP-glucuronate:xylan alpha-glucuronosyltransferase 1; UDP-GlcA:xylan glucuronyltransferase 1; Glycogenin-like protein 1; Plant glycogenin-like starch initiation protein 1; Protein GLUCURONIC ACID SUBSTITUTION OF XYLAN 1; AtGUX1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-659
Target Protein Sequence
MANSPAAPAPTTTTGGDSRRRLSASIEAICKRRFRRNSKGGGRSDMVKPFNIINFSTQDK NSSCCCFTKFQIVKLLLFILLSATLFTIIYSPEAYHHSLSHSSSRWIWRRQDPRYFSDLD INWDDVTKTLENIEEGRTIGVLNFDSNEIQRWREVSKSKDNGDEEKVVVLNLDYADKNVT WDALYPEWIDEEQETEVPVCPNIPNIKVPTRRLDLIVVKLPCRKEGNWSRDVGRLHLQLA AATVAASAKGFFRGHVFFVSRCFPIPNLFRCKDLVSRRGDVWLYKPNLDTLRDKLQLPVG SCELSLPLGIQDRPSLGNPKREAYATILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDD NISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYDKIIFIDADLL ILRNIDFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLN EVFTWWHRIPKHMNFLKHFWIGDEDDAKRKKTELFGAEPPVLYVLHYLGMKPWLCYRDYD CNFNSDIFVEFATDIAHRKWWMVHDAMPQELHQFCYLRSKQKAQLEYDRRQAEAANYADG HWKIRVKDPRFKICIDKLCNWKSMLRHWGESNWTDYESFVPTPPAITVDRRSSLPGHNL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Glycosyltransferase required for the addition of both glucuronic acid and 4-O-methylglucuronic acid branches to xylan in stem cell walls. In association with GUX2, is responsible for almost all of the substitutions of the xylan backbone in stem glucuronoxylan.
Gene References into Functions
  1. GUX1 and GUX2 are required for different patterns of 4-O-methylglucuronic acid substitution of xylan. PMID: 23373848
  2. GUX2 and GUX4, have activity as xylan alpha-glucuronosyltransferases. PMID: 22706449
  3. GUX1/2/3 are glucuronyltransferases responsible for the glucuronic acid substitution of xylan, which is essential for normal secondary wall deposition and plant development. PMID: 22537759
  4. Data show that a mutant in the PGSIP1 gene encoding one of the Golgi localized candidate proteins has a highly decreased content of glucuronic acid in secondary cell walls and substantially reduced xylan glucuronosyltransferase activity. PMID: 21124849
  5. Data identify two Golgi-localized glycosyltransferases, GlucUronic acid substitution of Xylan (GUX)-1 and GUX2, that are required for addition of glucuronic acid and 4-O-methylglucuronic acid branches to xylan in Arabidopsis cell walls. [GUX1] PMID: 20852069

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Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 8 family, Glycogenin subfamily
Database Links

KEGG: ath:AT3G18660

STRING: 3702.AT3G18660.2

UniGene: At.6378

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