Recombinant Danio rerio N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (gnptab)

Code CSB-CF732829DIL
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Source in vitro E.coli expression system
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Product Details

Target Names
gnptab
Uniprot No.
Alternative Names
gnptab; gnpta; si:ch211-234f20.3; zgc:122985; N-acetylglucosamine-1-phosphotransferase subunits alpha/beta; GlcNAc-1-phosphotransferase subunits alpha/beta; Stealth protein gnptab; UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
894-1219
Target Protein Sequence
DTFADSLRYVNRLLNAQFGFTSRKVPAHMPHMIDRLIMQELQDTFPQEFDKTSSHRVRHS EDMQFAFSYFYFLMSAVQQLNISEVFDEIDTDHSGVLSDREIRTLATRIHELPLSLQDLT SLEQMLINCSKSLPSNLTHLHAVSPTQEAYYDPSMPPVTKGLVIHCKPITERIHKAFKDQ NKYKFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFICLNDNIDHIHKDAGTVKAVL RDFYESMFPLPSQFELPREYRNRFLHMTELQEWRIYRDKLKFWTHCVLVTLVVFTVMSFF AEQLVMLKRWLFPRRRVSKDANPERV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.
Gene References into Functions
  1. The DMAP interaction domain of the alpha subunit functions in the selective recognition of acid hydrolase substrates and provides an explanation for the impaired phosphorylation of acid hydrolases in a patient with mucolipidosis II. PMID: 23733939
Subcellular Location
[N-acetylglucosamine-1-phosphotransferase subunit alpha]: Golgi apparatus membrane; Single-pass type I membrane protein.; [N-acetylglucosamine-1-phosphotransferase subunit beta]: Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Stealth family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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