Recombinant Danio rerio Transmembrane protein 8C (tmem8c)

Code CSB-CF743585DIL
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
mymk
Uniprot No.
Alternative Names
mymk; tmem8c; zgc:86927; Protein myomaker; Myoblast fusion maker; Transmembrane protein 8C
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-220
Target Protein Sequence
MGAFIAKMLLPTISSLVFVPAASVAAKRGFHMEAMVYFFTMFFTAIYHACDGPGLSILCF MKYDILEYFSVYGTAISMWVTLLALGDFDEPKRSSLTMFGVLTAAVRIYQDRLGYGIYSG PIGTAVFMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVH SFYHVSLAMSFILLLPKKNRYAGTGRNAAKLNCYTLCCCV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Myoblast-specific protein that mediates myoblast fusion, an essential step for the formation of multi-nucleated muscle fibers. Actively participates in the membrane fusion reaction by mediating the mixing of cell membrane lipids (hemifusion) upstream of mymx.
Gene References into Functions
  1. The data establish that MYMK activity is necessary for normal muscle development and maintenance in humans and zebrafish, and expand the spectrum of congenital myopathies to include cell-cell fusion deficits. PMID: 28681861
  2. These findings underscore a conserved role for Myomaker in vertebrate myocyte fusion. PMID: 28161523
  3. Confocal microscopy showed marked phenotype characterized by persistence of mononucleated muscle cells in fast myotome at developmental stages where these cells normally fuse to form multinucleated myotubes. myomaker is essential for myocyte fusion. PMID: 25078621
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
TMEM8 family
Database Links

KEGG: dre:415178

UniGene: Dr.36067

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