Recombinant Drosophila melanogaster G-protein coupled receptor moody (moody)

Code CSB-CF896194DLU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
moody
Uniprot No.
Alternative Names
moody; CG4322; G-protein coupled receptor moody
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-670
Target Protein Sequence
MSDETTISLEDGYPPLEALTTMVPPADATGFSQSLLTFAAVMTFLIMIVGICGNLLTVVA LLKCPKVRNVAAAFIISLCIADLLFCALVLPFQGLRFVQGTWRHGQVLCRLIPFIQYGNI GVSLLCIAMITINRYVMITHHGLYARIYKRHWIAVMIAACWLFSYGMQLPTLLGEWGRFG YDSRLQTCSIMTDDHGHSSKTTLFITAFVIPCLVIIACYAKIFWVVHKSEQRLKRHATKQ NSIPNNLRPLASTGSGALPSGAECQPSNRVSSDSSSSFSIDVPETAPSGKQQPTRVKDQR EVRAKRNEWRITKMVLAIFLSFVVCYLPITIVKVADKNVEHPSLHICSYILLYLSACINP IIYVIMNKQYRKAYKTVVFCQPARLLLPFGKTNGASSAAEKWKDTGLSNNHSRTIVSQMS GGTGAASGAGTATGTAAVAVMQTPPEVQQAQALEMVSRGPDLISKSNLPQPNVTPPPPSV LTATPNGSNSNSLTLRLPLKKNNHCYTNSGFNSSTPSPSSGLGIGISSSSIYRPGVGSLG SGSASIRRITMVGDDIILEEEELPPTPPATSAPTTPAPPPPSSPLHPLSTDSSTTTISGG AVVAGSSAPKPATPTPHIYMNVDSPKRNQYYMDRNTNAVAPESDSGPANTSATVSISGSK LTAKMKFPKD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Isoform A and isoform B are required in glia to regulate the acute sensitivity to cocaine and to continuously maintain the proper blood-brain barrier (BBB) function. A moody-mediated signaling pathway functions in glia to regulate nervous system insulation and drug-related behaviors. Galphai and Galphao, and the regulator of G protein signaling, loco, are required in the surface glia to achieve effective insulation. The components function by regulating the cortical actin and thereby stabilizing the extended morphology of the surface glia, which in turn is necessary for the formation of septate junctions of sufficient length to achieve proper sealing of the nerve cord.
Gene References into Functions
  1. The results suggest a mechanism by which the Drosophila BBB is maintained by Moody/GPCR-dependent formation of ARSs, which is supported by myosin activation. PMID: 21242289
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Isoform A and isoform B are expressed in the head. Isoform B only is expressed in the body. Expressed in embryonic glial cells that are involved in ensheathment and insulation of the nervous system. Both isoforms are expressed in glia that insulate the la
Database Links

KEGG: dme:Dmel_CG4322

STRING: 7227.FBpp0292162

UniGene: Dm.12246

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