Recombinant Drosophila melanogaster Zinc transporter foi(foi)

Code CSB-CF895187DLU
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names foi
Uniprot No. Q9VSL7
Alternative Names foi; CG6817; Zinc transporter foi; Protein fear-of-intimacy; Protein kastchen
Species Drosophila melanogaster (Fruit fly)
Expression Region 22-706
Target Protein Sequence QDHIESLLGPARVTTHNSQDQLNARVYTNLSPSSETTDRRQQRSASGDDDTFNYSISPPS RREKRHAGHEHGPTSESRVPQITQYYLEKLMAQDELMNSSGFDGLLQQLSLHSLASGASE GTCVPGSRLVHHVQPHDHHHAHHHEEEDHSLQLNNCTLIQNGTTSNVICPSLPNNNTHPL GKEAKNFTLSDKDLLHLCPILLYELKAQSGGCIEPAILSDIDTTEELLEAEKDKDIFYVW IYAFISVFACGILGLVGVAIIPFMGSRYYKYIIQYLVALAVGTMTGDALLHLLPHSLAGQ DERGMIMKGLGCLGGIIFFYVMEHALTMISEWRKSVEKKETKKPSRAKVMRDPDSSVNNS VAGDKICKQKYSSYPYCYDEITMNNKQSEWMHLPFDVAAGAGGDAPSVAELRNGVGDHDG SNDMAAAAESLISPLHTNCVEMNHHNHNHKHNSHQQNHEGQDSNTIVTDLDGNAVYAVNK AKDKDSRNDHVTVILREHESSHHGHSHRHGHVHSPPETLSAVAWMIIMGDGLHNFTDGMA IGAAFAENIAGGFSTSLAVFCHELPHELGDFAILIKAGMSVKSAVYYNLLTGVLSFIGMI FGIAFGQSQDVAQWMFAVAAGLFIYIALVDMMPEISASHKSLGQFLLQILGMLSGVGIML LIALYEGDLMSAFGTAGAASHQHAH
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Required for the normal migration of longitudinal and peripheral glial cells. During larval development, required for the migration of the subretinal glia into the eye disk. During embryonic development, also controls the migration of muscle cells toward their attachment sites. Required in the mesoderm for the correct morphogenesis of embryonic gonad and for tracheal branch fusion during tracheal development. Shg may be cooperating with foi to mediate a common mechanism for gonad and tracheal morphogenesis. Acts as a zinc transporter in both yeast and mammalian cells.
Gene References into Functions
  1. ZIP transporter Fear-of-intimacy (Foi) is necessary for the formation of Drosophila muscles. PMID: 27068109
  2. cell-surface protein required in the mesoderm for gonad morphogenesis PMID: 12702650
  3. in vivo structure-function analysis of FOI that strongly indicates that zinc transport activity of FOI is essential for gonad development PMID: 16481356
Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families ZIP transporter (TC 2.A.5) family
Tissue Specificity Maternal foi has almost completely disappeared by embryonic stage 3 except in the pole cells. In stage 6 embryos, expression is enriched in the invaginating mesoderm. In stage 9 embryos, high levels in the anterior and posterior midgut primordia. In stage
Database Links

KEGG: dme:Dmel_CG6817

STRING: 7227.FBpp0076350

UniGene: Dm.2818

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