Recombinant Friend spleen focus-forming virus Glycoprotein 55 (env)

Code CSB-CF365928FDD
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
env
Uniprot No.
Alternative Names
env; Glycoprotein 55; gp55
Species
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Expression Region
33-409
Target Protein Sequence
VQHDSPHQVFNVTWRVTNLMTGQTANATSLLGTMTDAFPMLHFDLCDLIGDDWDETGLEC RTPGGRKRARTFDFYVCPGHTVPTGCGGPREGYCGKWGCETTGQAYWKPSSSWDLISLKR GNTPKDRGPCYDSSVSSGVQGATPGGRCNPLVLKFTDAGKKASWDSPKVWGLRLYRPTGI DPVTRFSLTRQVLNIGPRIPIGPNPVIIGQLPPSRPVQVRLPRPPQPPPTGAASMVPGTA PPSQQPGTGDRLLNLVQGAYQALNLTNPDKTQECWLCLVSGPPYYEGVAVLGTNSNHTSA LKEKCCFYADHTGLVRDSMAKLRKRLTQRQKLFESSQGWFEGSFNRSPWFTTLISTIMGL LIILLLLLILLLWTLHS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform of MST1R receptor tyrosine kinase (MST1R isoform sf-Stk). Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk.
Subcellular Location
Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein. Note=The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity.
Database Links

KEGG: vg:1491889

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