Recombinant Human Diacylglycerol kinase epsilon(DGKE)

Code CSB-CF006835HU
Size US$3809Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names DGKE
Uniprot No. P52429
Research Area Signal Transduction
Alternative Names AHUS7; DAG kinase epsilon; DAGK 5; DAGK 6; DAGK5; DAGK6; DGK; DGK epsilon; DGK-epsilon; DGKE; DGKE_HUMAN; Diacylglycerol kinase epsilon 64kDa; Diacylglycerol kinase epsilon; Diglyceride kinase epsilon; NPHS7
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 1-567aa
Target Protein Sequence MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQEDIKATE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 83.9kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Gene References into Functions
  1. mutations can lead to atypical hemolytic uremic syndrome or membranoproliferative glomerulonephritis PMID: 28526779
  2. Letter/Case Report: atypical haemolytic uraemic syndrome in a Japanese patient with DGKE genetic mutations. PMID: 26018111
  3. Report DGKE intronic mutations located beyond the exon-intron boundaries in familial hemolytic uremic syndrome. PMID: 25854283
  4. Data suggest that complement dysregulation influences the onset and disease severity in carriers of diacylglycerol kinase-epsilon mutations PMID: 25135762
  5. DGKE silencing in resting endothelial cells does not affect complement activation at their surface. PMID: 25498910
  6. Our study expands the clinical phenotypes associated with mutations in DGKE and challenges the benefits of complement blockade treatment in such patients PMID: 24511134
  7. DGKzeta localizes to the nucleus and is considered to regulate nuclear diacylglycerol signaling.[review] PMID: 24119575
  8. Fully activating high-density transfected muscarinic receptors (M1Rs) by oxotremorine-M (Oxo-M) leads to similar calcium, DAG, and PKC signals, but PIP2 is depleted. PMID: 23630338
  9. Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. PMID: 23542698
  10. Substrate specificity of DGKE is not a consequence of competition with hydrolysis of ATP. PMID: 23261795
  11. performed homozygosity mapping and whole exome sequencing in a Turkish consanguineous family and identified DGKE gene variants as the cause of a membranoproliferative-like glomerular microangiopathy PMID: 23274426
  12. Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity. PMID: 22511757
  13. A role for diacylglycerol kinase (DGK) and its downstream product phosphatidic acid (PA) in ANCA-induced neutrophil exocytosis, is reported. PMID: 21833457
  14. The region responsible for this arachidonoyl specificity is the lipoxygenase (LOX)-like motif found in the accessory domain, adjacent to DGKvarepsilon's catalytic site. PMID: 22266092
  15. diacylglycerol kinase-epsilon (DGKepsilon) has less preference for the acyl chain at the sn-1 position of diacylglycerol (DAG) than the one at the sn-2 position PMID: 22108654
  16. DGK activity is reduced by oxidative stress in human mesangial cells cultured under high glucose conditions. PMID: 21725595
  17. The hydrophobic domain of diacylglycerol kinase epsilon does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane. PMID: 17455907
  18. The alpha and zeta isoforms of diacylglycerol kinase are inhibited by 2,3-dioleoylglycerol, but not the more substrate-selective epsilon isoform PMID: 18004883
  19. Substrate specificity of diacylglycerol kinase epsilon is determined by selectivity of the sn-1 and sn-2 acyl chains phosphatidic acid or diacylglycerol. PMID: 19744926

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Involvement in disease Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7)
Subcellular Location Membrane, Multi-pass membrane protein, Membrane, Cytoplasm
Protein Families Eukaryotic diacylglycerol kinase family
Tissue Specificity Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
Database Links

HGNC: 2852

OMIM: 601440

KEGG: hsa:8526

STRING: 9606.ENSP00000284061

UniGene: Hs.239514

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