DGKE Antibody

Code CSB-PA006835ESR1HU
Size US$166
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  • Western blot
    All lanes: Diacylglycerol kinase epsilon antibody at 5μg/ml
    Lane 1: Rat brain tissue
    Lane 2: Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 64, 21 kDa
    Observed band size: 64 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA006835ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA006835ESR1HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DGKE Polyclonal antibody
Uniprot No.
Target Names
DGKE
Alternative Names
DGKE; DAGK5; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Diacylglycerol kinase epsilon protein (40-260AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.
Gene References into Functions
  1. mutations can lead to atypical hemolytic uremic syndrome or membranoproliferative glomerulonephritis PMID: 28526779
  2. Letter/Case Report: atypical haemolytic uraemic syndrome in a Japanese patient with DGKE genetic mutations. PMID: 26018111
  3. Report DGKE intronic mutations located beyond the exon-intron boundaries in familial hemolytic uremic syndrome. PMID: 25854283
  4. Data suggest that complement dysregulation influences the onset and disease severity in carriers of diacylglycerol kinase-epsilon mutations PMID: 25135762
  5. DGKE silencing in resting endothelial cells does not affect complement activation at their surface. PMID: 25498910
  6. Our study expands the clinical phenotypes associated with mutations in DGKE and challenges the benefits of complement blockade treatment in such patients PMID: 24511134
  7. DGKzeta localizes to the nucleus and is considered to regulate nuclear diacylglycerol signaling.[review] PMID: 24119575
  8. Fully activating high-density transfected muscarinic receptors (M1Rs) by oxotremorine-M (Oxo-M) leads to similar calcium, DAG, and PKC signals, but PIP2 is depleted. PMID: 23630338
  9. Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. PMID: 23542698
  10. Substrate specificity of DGKE is not a consequence of competition with hydrolysis of ATP. PMID: 23261795
  11. performed homozygosity mapping and whole exome sequencing in a Turkish consanguineous family and identified DGKE gene variants as the cause of a membranoproliferative-like glomerular microangiopathy PMID: 23274426
  12. Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity. PMID: 22511757
  13. A role for diacylglycerol kinase (DGK) and its downstream product phosphatidic acid (PA) in ANCA-induced neutrophil exocytosis, is reported. PMID: 21833457
  14. The region responsible for this arachidonoyl specificity is the lipoxygenase (LOX)-like motif found in the accessory domain, adjacent to DGKvarepsilon's catalytic site. PMID: 22266092
  15. diacylglycerol kinase-epsilon (DGKepsilon) has less preference for the acyl chain at the sn-1 position of diacylglycerol (DAG) than the one at the sn-2 position PMID: 22108654
  16. DGK activity is reduced by oxidative stress in human mesangial cells cultured under high glucose conditions. PMID: 21725595
  17. The hydrophobic domain of diacylglycerol kinase epsilon does not contribute to substrate specificity but plays a role in permanently sequestering the enzyme to a membrane. PMID: 17455907
  18. The alpha and zeta isoforms of diacylglycerol kinase are inhibited by 2,3-dioleoylglycerol, but not the more substrate-selective epsilon isoform PMID: 18004883
  19. Substrate specificity of diacylglycerol kinase epsilon is determined by selectivity of the sn-1 and sn-2 acyl chains phosphatidic acid or diacylglycerol. PMID: 19744926

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Involvement in disease
Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7)
Subcellular Location
Membrane; Single-pass membrane protein. Cytoplasm.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
Database Links

HGNC: 2852

OMIM: 601440

KEGG: hsa:8526

STRING: 9606.ENSP00000284061

UniGene: Hs.239514

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