Recombinant Human Endothelin-converting enzyme 1(ECE1)

Code CSB-CF007371HU
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names ECE1
Uniprot No. P42892
Alternative Names ECE1Endothelin-converting enzyme 1; ECE-1; EC 3.4.24.71
Species Homo sapiens (Human)
Expression Region 1-770
Target Protein Sequence MRGVWPPPVSALLSALGMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Converts big endothelin-1 to endothelin-1.
Gene References into Functions
  1. ECE-1 and ECE-2 levels were significantly reduced in post-mortem brains from dementia with Lewy body patients. PMID: 28171705
  2. These results indicated that the ECE1 rs212528 and rs213045 polymorphisms had no major role to play in the genetic susceptibility to intracerebral haemorrhage. PMID: 27036146
  3. results indicate that PKC-phosphorylated ECE-1 is a TIMAP-PP1c substrate and this phosphatase complex has an important role in endothelin-1 production of EC through the regulation of ECE-1 activity. PMID: 26806547
  4. Thus, our findings suggest that ECE-1 may be a novel hypoxia-inducible factor-target gene. PMID: 25469848
  5. Structural analysis of membrane-bound hECE-1 dimer using molecular modeling techniques: insights into conformational changes and Abeta1-42 peptide binding. PMID: 25501500
  6. Nitric oxide inhibits the production of soluble endothelin converting enzyme-1 by endothelial cells. PMID: 25226840
  7. confirmed that endothelin-converting enzyme 1 (ECE1), EDN1, and EDNRB were involved in SP-induced pigmentation and found that EDN1 secretion was affected by ECE1 and EDN1 siRNAs, but not by EDNRB siRNA PMID: 25268585
  8. An arterial-specific enhancer of the human endothelin converting enzyme 1 (ECE1) gene is synergistically activated by Sox17, FoxC2, and Etv2 PMID: 25179465
  9. Compared with rs213045 G homozygote, rs213045 TG genotype and rs213045 TT/TG genotypes are in dominant model significantly increased the risk of ischemic stroke. PMID: 24595843
  10. Although the presence of a soluble form of ECE-1 has been demonstrated in vitro, it is yet to be shown in any human biological fluid. This study, used a combination of mass spectrometry, Western blotting & quenched fluorescent substrate based enzyme assays & showed presence of catalytically active, soluble form of ECE-1 in CSF of subarachnoid hemorrhage subjects. PMID: 23816989
  11. Data indicate endothelin-converting enzyme-1 (ECE-1) as a target for alternative polyadenylation (APA), a regulatory mechanism aberrantly activated in cancer cells, and the mechanisms leading to ECE-1 overexpression in malignant cells. PMID: 24497914
  12. the primary function of betaARRs and ECE-1 in SP-dependent inflammatory signaling is to promote resensitization, which allows the sustained NK1R signaling from the plasma membrane that drives inflammation PMID: 24898255
  13. phosphoramidon and Abeta peptide binding to hECE-1 PMID: 24000822
  14. identify the neural peptidase ECE-1 as a negative regulator of itch on sensory nerves by directly regulating ET-1-induced pruritus PMID: 24812665
  15. study suggests that the severity of periodontal disease may be associated with the expression of metalloendopeptidase genes, including NEP, ECE1 and ADAM17, in the buccal mucosal epithelium. PMID: 23360525
  16. C-338A polymorphism of the ECE-1b gene was associated with an increased risk of ischemic stroke in the Chinese Han population. PMID: 24026618
  17. The altered expression of enzymes regulating the activity of ET-1, NEP and ECE-1, during parturition is controlled by inflammatory cytokines. PMID: 23863409
  18. Endothelin-converting enzymes degrade intracellular beta-amyloid produced within the endosomal/lysosomal pathway and autophagosomes PMID: 23283972
  19. The Alzheimer's disease associated intronic haplotype is linked to the 338A variant of known ECE1b promoter variant, 338C>A (rs213045). PMID: 22693153
  20. The results complete a gap in our understanding of the mechanism(s) behind PKC induced trafficking of ECE-1. PMID: 23022525
  21. involved in the physiological degradation of atrial natriuretic peptide PMID: 22972025
  22. we demonstrate that a CpG-CA repeat within the human ECE-1c promoter is highly polymorphic, harbors transcriptional start sites PMID: 23175834
  23. review of ECE-1 phosphorylation and other stimuli which act induce trafficking of ECE-1 to the cell surface [review] PMID: 22728136
  24. results suggest that ECE1 polymorphisms may contribute to the susceptibility of sporadic congenital heart disease in a Chinese population PMID: 22731820
  25. Endothelin-converting enzyme-1 (ECE-1) degrades NT in acidic conditions, and its activity is crucial for NTR1 recycling. PMID: 22416137
  26. Our results point to a regulatory function of ET-1, its type B receptor (ET(B)) and endothelin-converting enzyme-1 proteins in transendothelial passage of monocytes PMID: 21777246
  27. Te results of this study suggested taht genetic variations in MMEL1, ECE1, ECE2, AGER, PLG, PLAT, NR1H3, MMP3, LRP1, TTR, NR1H2, and MMP9 genes do not play major role among the Finnish AD patient cohort. PMID: 22027013
  28. 4,4'-dihydroxy-trans-stilbene strongly inhibits ECE-1 activity, whereas resveratrol is inactive. PMID: 21554123
  29. Results of this study suggest lack of direct correlation of Lys198Asn ET-1 and Thr341lle ECE-1 gene polymorphisms with risk of gestational hypertension and preeclampsia in the studied population of Polish women PMID: 21851036
  30. any disease-specific contribution of ECE-1 to the accumulation of Abeta or reduction in local microvascular blood flow in Alzheimer disease or Vascular dementia is probably small. PMID: 20345647
  31. ECE-1 influences prostate cancer cell invasion via both ET-1-mediated FAK phosphorylation and ET-1 independent mechanisms. PMID: 20725143
  32. ECE-1 is not correlated with amyloig beta or clinical diagnosis Alzheimer disease PMID: 20663017
  33. Data show that CD133 and ECE expressions are associated with lymphoid metastasis and prognosis of NSCLC, and their overexpression often suggests unfavorable prognosis of NSCLC. PMID: 17545092
  34. These data indicate for the first time that PKC activation induces the trafficking and shedding of ECE to and from the cell surface, respectively. PMID: 20558134
  35. study argues that the ECE1 338A allele is protective against late-onset Alzheimer disease in a Chinese population PMID: 20037208
  36. ECE-1alpha is expressed at significant levels in various types of human skin cells (including keratinocytes) and that it plays a constitutive role in the processing and UVB-inducible secretion of ET-1 by human keratinocytes PMID: 11723113
  37. identification of potential residues involved in phosphorylation PMID: 12244060
  38. Data demonstrate that the targeting signals specific for endothelin-converting enzyme 1b constitute a regulatory domain that could modulate the localization and the activity of other isoforms. PMID: 12393864
  39. ECE1 has a role in limiting Abeta accumulation in the mouse brain PMID: 12464614
  40. It is suggested that endothelial cells sense shear stress as oxidative stress and transduce signal for the regulation of the gene expression of ECE. PMID: 12609744
  41. Cis-acting elements bind CAAT-box binding protein NF-YB, GATA-2, E2F-2, and a GC-box binding factor associated with transcriptional start sites of ECE-1c. Three polymorphic dinucleotide repeats. Methylation suppressed ECE-1c promoter in endothelium. PMID: 14597855
  42. The main isoform to increase in response to high glucose was ECE-1c and it may be one of the factors contributing to the elevated ET-1 peptide levels observed in diabetes. PMID: 15010576
  43. This EVA-based study suggests that ECE1 interacts with ET1 to influence blood pressure in women. PMID: 15126915
  44. Neuronal ECE-1 expression was observed in various cortical regions of nondemented subjects. In a case-control study involving patients with late-onset AD, homozygous carriers of the A allele of ECE1 gene had a reduced risk of AD. PMID: 15340356
  45. locally synthesized AII could be one of the mediators involved in the down-regulation of ECE-1 PMID: 15665524
  46. ECE-1 protein expression, prepro-endothelin-1 mRNA, and endothelin-1 peptide release were increased in response to native LDL or oxidized LDL. PMID: 16023075
  47. Observations strongly suggest that the expression of ECE is enhanced in neointimal smooth muscle cells at early stages after percutaneous coronary intervention injury in human coronary arteries. PMID: 16531800
  48. increased neuronal PKCepsilon activity can promote Abeta clearance and reduce AD neuropathology through increased endothelin-converting enzyme activity PMID: 16698938
  49. These findings suggest that endothelin-converting enzyme (ECE) is upregulated in the neointima at early stages after percutaneous coronary intervension (PCI) injury. ECE may be one of the mediators in the repair processes after PCI in humans. PMID: 16986361
  50. Homozygous carriers of the ECE-1 -839G variant allele exhibited a decreased risk of coronary artery disease PMID: 17264805
  51. Endosomal peptidase ECE-1 degrades neuropeptides in endosomes to disrupt the peptide-receptor-beta-arrestin complex, thereby controlling postendocytic trafficking and signaling of receptors. PMID: 17592116
  52. ECE-1b-338C to A variant might be associated with increased risk of CAD in Chinese population. PMID: 17618613
  53. genotyped 5 single nucleotide polymorphisms in the ECE1 gene in 1,873 individuals from a general Japanese population and identified one associated with hypertension in women PMID: 17664854
  54. Endothelin-1 and endothelin-converting enzyme-1 have roles in human granulomatous pathology of eyelid PMID: 17701914
  55. Spacio-temporal expression of two endopeptidases, ECE-1 and NEP, involved in the synthesis and degradation of ET-1, might regulate ET-1 action in human endometrium. PMID: 17712175
  56. Results show catalytically active ECE-1 was detected in the media of human umbilical vein endothelial cells, and was confirmed by mass spectrometry based assays. PMID: 17761169
  57. A mechanism by which endosomal ECE-1 degrades neuropeptides in endosomes to disrupt the peptide/receptor/beta-arrestin complex, freeing internalized receptors from beta-arrestins and promoting recycling and resensitization, is proposed. PMID: 18039931
  58. A allele was less present in LOAD patients than in controls, but an at limits statistically significant difference was achieved only in subjects aged less than 80 years PMID: 18334739
  59. Transient ECE-1c overexpression increased PC-3 invasiveness through matrigel, whereas transient ECE-1a expression suppressed invasion PMID: 18781169
  60. Enhanced ECE-1 expression induced by hyperglycemia is partly due to activation of the PKC-delta isoform. Thus, inhibition of this PKC isoform may prevent diabetes-related increase in ET-1. PMID: 18974277
  61. The crystal structure of the extracellular domain (residues 90-770) of human ECE-1 (C428S) with the generic metalloprotease inhibitor phosphoramidon determined at 2.38 A resolution. PMID: 18992253
  62. By degrading endocytosed SP, ECE-1 promotes the recycling and re-sensitization of NK(1) receptors in endothelial cells, and thereby induces re-sensitization of the pro-inflammatory effects of SP. PMID: 19222484
  63. C-338 polymorphism of the ECE1 gene might be associated with increased risk of carotid atherosclerosis in the Chinese population. PMID: 19289136
  64. Endosomal endothelin-converting enzyme-1 is a regulator of beta-arrestin-dependent ERK signaling PMID: 19531493

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Involvement in disease Hirschsprung disease, cardiac defects, and autonomic dysfunction (HCAD)
Subcellular Location Cell membrane, Single-pass type II membrane protein
Protein Families Peptidase M13 family
Tissue Specificity All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A an
Database Links

HGNC: 3146

OMIM: 600423

KEGG: hsa:1889

STRING: 9606.ENSP00000364028

UniGene: Hs.195080

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