Recombinant Human Phosphatidate phosphatase PPAPDC1B (PPAPDC1B)

Code CSB-CF836728HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
PLPP5
Uniprot No.
Alternative Names
PLPP5; DPPL1; HTPAP; PPAPDC1B; Phospholipid phosphatase 5; Phosphatidic acid phosphatase type 2 domain-containing protein 1B
Species
Homo sapiens (Human)
Expression Region
1-264
Target Protein Sequence
MGKAAAAVAFGAEVGVRLALFAAFLVTELLPPFQRLIQPEEMWLYRNPYVEAEYFPTKPM FVIAFLSPLSLIFLAKFLKKADTRDSRQACLAASLALALNGVFTNTIKLIVGRPRPDFFY RCFPDGLAHSDLMCTGDKDVVNEGRKSFPSGHSSFAFAGLAFASFYLAGKLHCFTPQGRG KSWRFCAFLSPLLFAAVIALSRTCDYKHHWQDVLVGSMIGMTFAYVCYRQYYPPLTDAEC HKPFQDKLVLSTAQKPGDSYCFDI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Magnesium-independent phospholipid phosphatase with broad substrate specificity. Preferentially catalyzes the conversion of diacylglycerol pyrophosphate into phosphatidate but can also act on phosphatidate and lysophosphatidate. Phospholipid phosphatases are involved in both the synthesis of lipids and the generation or degradation of lipid-signaling molecules.
Gene References into Functions
  1. genetic variants at loci -1053 and +64 of the HTPAP promoter affect the expression of HTPAP, which might be a novel determinant and target for HCC prognosis PMID: 24603412
  2. Findings identify an HTPAP genotype and associated gene expression pattern that favors metastasis progression and that could be used to predict tumor metastasis and prognosis in HCC patients. PMID: 21531764
  3. HTPAP-1 may be the transcript of HTPAP to exhibit a suppressive role on hepatocellular cancer(HCC) metastasis, and can be a prognostic marker for HCC. PMID: 21219541
  4. HTPAP is a novel metastatic suppressor gene for hepatocellular carcinoma PMID: 16261160
  5. DPPL1 and DPPL2 represent a novel type of mammalian phosphatidate phosphatase. PMID: 17590538
  6. microarray experiments on PPAPDC1B knockdown indicated this gene interfered with cell signaling pathways, including Janus-activated kinase-signal transducer and activator of transcription, mitogen-activated protein kinase, and protein kinase C pathways PMID: 18757432

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
PA-phosphatase related phosphoesterase family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 25026

OMIM: 610626

KEGG: hsa:84513

STRING: 9606.ENSP00000392553

UniGene: Hs.567619

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