Recombinant Human Protein orai-3 (ORAI3)

Code CSB-CF887111HU
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
ORAI3
Uniprot No.
Alternative Names
ORAI3; TMEM142C; Protein orai-3; Transmembrane protein 142C
Species
Homo sapiens (Human)
Expression Region
1-295
Target Protein Sequence
MKGGEGDAGEQAPLNPEGESPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAK LKASSRTSALLSGFAMVAMVEVQLESDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLL PHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAP LDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGP GWQAAMASTAIMVPVGLVFVAFALHFYRSLVAHKTDRYKQELEELNRLQGELQAV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1.
Gene References into Functions
  1. mRNA expression in differentiated keratinocytes increased with increasing age of donors, and the increase corresponded well to slower recovery of [Ca2+]i from hydraulic stress-induced elevation in keratinocytes of older donors PMID: 27571764
  2. These data suggest that Orai3 channel does not participate in the thapsigargin-revealed ER Ca(2+) leak but forms an ER Ca(2+) leak channel that is limiting the overloading with Ca(2+) of the ER store. PMID: 28179072
  3. Orai3 constitutes a native store-operated calcium entry pathway in non small cell lung adenocarcinoma that controls cell proliferation and cell cycle progression, likely via Akt pathway. PMID: 24058448
  4. High ORAI3 expression is associated with breast cancer. PMID: 23266555
  5. data establish Orai3 as an ERalpha-regulated channel and a potential selective therapeutic target for ERalpha(+) breast cancers PMID: 22993197
  6. the N-terminal conserved region plays a multifaceted role in Orai3 current gating with distinct structural requirements for STIM1- and 2-APB-stimulated activation. PMID: 21724845
  7. Results provide strong evidence for a significant effect of Orai3 on breast cancer cell growth in vitro and show that this effect is associated with the induction of cell cycle and apoptosis resistance. PMID: 20683915
  8. stimulation of Orai3 currents by 2-aminoethoxydiphenyl borate occurred along with an alteration of the permeation pathway that represents a unique mechanism for regulating ion channel selectivity by chemical compounds PMID: 18499656

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Orai family
Tissue Specificity
Expressed in both naive and effector T helper cells with higher levels in effector cells.
Database Links

HGNC: 28185

OMIM: 610930

KEGG: hsa:93129

STRING: 9606.ENSP00000322249

UniGene: Hs.745104

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