Recombinant Human Protein wntless homolog (WLS)

Code CSB-CF716359HU
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
WLS; C1orf139; GPR177; UNQ85/PRO18667; Protein wntless homolog; Integral membrane protein GPR177; Protein evenness interrupted homolog; EVI; Putative NF-kappa-B-activating protein 373
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
Full Length of Mature Protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
Gene References into Functions
  1. GPR177 as a novel candidate for prognostic marker as well as a promising target for treatment of gastric cancer patients. PMID: 29555015
  2. Wls is differentially expressed in Intrahepatic Cholangiocarcinoma tissues and positively related to tumor stage and lymphatic invasion. PMID: 29526886
  3. These data showed that Wls was differentially expressed in HCC. Statistical analysis results suggest that Wls expression might increase as HCC progresses. PMID: 29127606
  4. Wls-SEC12 binding is stable, with the interacting interface biochemically mapped to cytosolic segments of individual proteins. Mutant Wls that fails to communicate with the COPII machinery cannot effectively support Wnt secretion. These data suggest that formation of early Wnt secretory vesicles is carefully regulated to ensure proper export of functional ligands PMID: 28515233
  5. we identified novel associations in WLS , ARHGAP1 , and 5' of MEF2C ( P- values < 8x10 - 5 ; false discovery rate (FDR) q-values < 0.01) that were much more strongly associated with BMD compared to the GWAS SNPs. PMID: 27616567
  6. Our data suggest that Wls protein is related to tumor metastasis and advanced TNM stage, and may be used as a new marker for prognosis of gastric carcinoma. PMID: 28110490
  7. These results indicate that WLS may play a role in invasion and metastasis of colorectal carcinoma. PMID: 27102079
  8. Dysfunction of Wntless triggers the retrograde Golgi-to-ER transport of Wingless and induces ER stress. PMID: 26887613
  9. Genetic variation at the WLS and CCDC170/ESR1 loci were found to be significantly associated with bone mineral density PMID: 26911590
  10. This study has revealed a strong association between the expression of WLS and HER2 that has important biological and clinical implications. PMID: 25258105
  11. This study identified CMTM8 as a new candidate tumor suppressor gene and GPR177 as a new candidate oncogene in osteosarcoma. PMID: 25551557
  12. GPR177 played an essential role in disease relapse and poor survival in patients with B-cell precursor acute lymphoblastic leukemia. PMID: 25115440
  13. endogenous WLS binds Wnts in the endoplasmic reticulum, cycles to the plasma membrane, and then returns to the endoplasmic reticulum through the Golgi. PMID: 24768165
  14. Colorectal tumors express elevated levels of Wnt3 and GPR177. PMID: 24162018
  15. The data suggest that the common variants of WLS analyzed in this study are not associated with opioid or cocaine addiction. PMID: 23566366
  16. Evi expression in psoriatic skin biopsies is down-regulated, suggesting that Evi-deficient mice developed skin lesions that resemble human psoriasis. PMID: 23918954
  17. findings lead to a proposed mechanism by which Gpr177 controls skeletal development through modulation of autocrine and paracrine Wnt signals in a lineage-specific fashion PMID: 23188710
  18. WLS functions as a negative regulator of melanoma proliferation and spontaneous metastasis by activating WNT/beta-catenin signalling. PMID: 23129487
  19. The Evi/Wls overexpression is sufficient to promote downstream Wnt signalling. PMID: 22147553
  20. Findings demonstrate that endocytosis motifs can occur in the intracellular loops of multipass membrane proteins and, moreover, provide direct evidence that the trafficking of Wls is required for efficient secretion of Wnt signaling proteins. PMID: 22027831
  21. The relatively ubiquitous expression of GPR177 suggests that this protein may serve to regulate Wnt secretion in a variety of embryonic and adult tissue types. PMID: 20652957
  22. These findings suggest a previously unrecognized role for GPR177 in regulating cellular response to opioid agonist drugs, and could create new avenues of investigation regarding the pharmacotherapy of drug dependence. PMID: 20214800

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Subcellular Location
Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.
Protein Families
Wntless family
Database Links

HGNC: 30238

OMIM: 611514

KEGG: hsa:79971

STRING: 9606.ENSP00000346829

UniGene: Hs.647659

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