Recombinant Human Solute carrier family 23 member 2(SLC23A2)

Code CSB-CF866221HU(A4)
Size How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names SLC23A2
Uniprot No. Q9UGH3
Research Area Metabolism
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 1-650aa
Target Protein Sequence MMGIGKNTTSKSMEAGSSTEGKYEDEAKHPAFFTLPVVINGGATSSGEQDNEDTELMAIYTTENGIAEKSSLAETLDSTGSLDPQRSDMIYTIEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGYDQWATSQLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTDVSVANGTAELLHTEHIWYPRIREIQGAIIMSSLIEVVIGLLGLPGALLKYIGPLTITPTVALIGLSGFQAAGERAGKHWGIAMLTIFLVLLFSQYARNVKFPLPIYKSKKGWTAYKLQLFKMFPIILAILVSWLLCFIFTVTDVFPPDSTKYGFYARTDARQGVLLVAPWFKVPYPFQWGLPTVSAAGVIGMLSAVVASIIESIGDYYACARLSCAPPPPIHAINRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGITKVGSRRVIQCGAALMLALGMIGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQNPLVTGITGIDQVLNVLLTTAMFVGGCVAFILDNTIPGTPEERGIRKWKKGVGKGNKSLDGMESYNLPFGMNIIKKYRCFSYLPISPTFVGYTWKGLRKSDNSRSSDEDSQATG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 71.3 kDa
Protein Length Full Length
Tag Info C-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na(+) for each ascorbate.
Gene References into Functions
  1. enhanced expression of the high affinity ascorbic acid transporter SVCT2, which tightly regulates intracellular ascorbic acid concentrations. PMID: 26646539
  2. mutant KRAS can be bypassed by L-ascorbic acid in an SVCT-2-dependent manner. Furthermore, SVCT-2 in mutant KRAS colon cancer may act as a potent marker for potentiating L-ascorbic acid co-treatment with cetuximab. PMID: 27012422
  3. These data suggest that both fruit intake and genetic marker in SLC23A2 may play an independent role in chronic lymphocytic leukemia biology. PMID: 26838684
  4. Ascorbic acid kills cholangiocarcinoma cells via DNA damage, ATP depletion, and inhibition of mTOR pathway in a SVCT-2 dependent manner. PMID: 28385602
  5. Our findings show, for the first time, that transporters of the water-soluble vitamin ascorbic acid (i.e., the vitamin C transporters SVCT-1 and SVCT-2) are differentially expressed along the length of the intestinal tract and that the pattern of expression is mediated, at least in part, by transcriptional and epigenetic mechanism(s) affecting both Slc23a1 and Slc23a2 genes. PMID: 27932501
  6. In title. PMID: 26188149
  7. Significant methylation changes in the SLC23A2 and NCOR2 regulatory regions. PMID: 25821969
  8. Vitamin C supplementation significantly increases skeletal muscle SVCT2 protein expression. PMID: 25242204
  9. ascorbic acid uptake mechanism, kinetics, and regulation by sodium dependent vitamin C transporter (SVCT2) in MDA-MB231, T47D and ZR-75-1 cells. PMID: 25102111
  10. Together, these data clarify previous inconsistencies in the literature andimplicate SVCT2 as the pericyte ascorbate transporter. PMID: 25645015
  11. The functional expression of SVCT2 was detected in HEK293 cells.The kinetic analysis suggested that an ascorbate-dependent mechanism accounts for targeted SVCT2 expression in the developing kidney during medullary epithelial cell differentiation. PMID: 22990596
  12. We propose that the mitochondrial localization of SVCT2 is a property shared across cells, tissues, and species. PMID: 24594434
  13. Demonstrate that the expression of SVCT2 transporter is significantly down-regulated in human grade 3 osteoarthritic tissues. PMID: 24401033
  14. polymorphisms in SLC23A1/2 genes influenced ascorbate concentration in aqueous humor and lens nucleus. PMID: 24815519
  15. Genetic variation in the sodium-dependent vitamin C transporter 2 may have a role in acute coronary syndrome in women PMID: 23990905
  16. Data show that the mRNA level of svct2 was approximately 600- to 900-fold higher than that of svct1 indicating SVCT2 is a main isoform in fibroblast OUMS-36 cells, and no significant difference in svct2 mRNA and protein between young and old cells. PMID: 23613229
  17. Low SVCT2 transporter is associated with Type I diabetes. PMID: 23999113
  18. Data suggest that N-terminal and C-terminal sorting signals interact, directly or indirectly, within each gene family (here, SVCT1 and SVCT2) in basolateral targeting of transmembrane proteins to basolateral cell membrane. PMID: 23837633
  19. confirmed the association between rs1279683 (SLC23A2) and primary open-angle glaucoma PMID: 23401652
  20. The novel demonstration of SVCT2-dependent mitochondrial transport of ascorbic acid. PMID: 23288661
  21. glutathione depletion failed to affect ascorbic acid transport, and SVCT1 and SVCT2 expression in hepatoma cells. Therefore, our data indicate an essential role for glutathione in controlling vitamin C metabolism in rat hepatocytes and rat hepatoma cells PMID: 22348976
  22. The rs1279683 single-nucleotide polymorphisms in SLC23A2 was significantly associated with lower plasma concentrations of vitamin C and with higher risk of primary open-angle glaucoma in GG subjects. PMID: 22171153
  23. In patients with hepatocellular cholestasis, primary biliary cirrhosis, haemochromatosis and non-alcoholic steatohepatitis, using real-time RT-PCR, an enhanced hepatic expression of both SLC23A1 and SLC23A2 was found. PMID: 21733302
  24. Thus CpG methylation at the upstream USF-binding site functions in establishing and maintaining cell-specific transcription from the CpG-poor SVCT2 exon 1a promoter. PMID: 21770893
  25. Differential occupancy of transcription factors on the GC-rich consensus sequences in the sodium-dependent vitamin C transporter (SVCT2) exon 1b promoter contributes to the regulation of cell and tissue expression of SVCT2. PMID: 21335086
  26. hSVCT1 and 2 promoters establish that ascorbic acid uptake by human liver epithelial cells is adaptively regulated and show that transcriptional mechanisms via HNF-1 in the hSVCT1 promoter may, in part, be involved in this regulation. PMID: 20471816
  27. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 19170196
  28. both NH(2)- and COOH-terminal sequences are essential for proper localization of hSVCT2, cell surface delivery is dependent on intact microtubules, and peripheral microfilaments regulate insertion and retrieval of hSVCT2 into the plasma membrane PMID: 19926816
  29. These findings suggest a mechanism of ascorbic acid uptake regulation whereby an alternative sodium-ascorbate cotransporter 2 (SVCT2) gene product inhibits transport through the two known ascorbic acid transporters. PMID: 15060139
  30. Functionally expressed in human endothelial cells and negatively regulated by inflammatory cytokines. May provide new insight into treatment of cardiovascular diseases with ascorbic acid. PMID: 15340249
  31. SVCT2 mediates the secondary active and concentrative transport of ascorbic acid in human chondrocytes PMID: 15921655
  32. Functionally, SVCT1 expression led to more transport activity from the apical membrane, while SVCT2 expression only increased the uptake under the condition when basolateral membrane was exposed. PMID: 15993839
  33. Findings link genetic variants in the vitamin C transporter gene SLC23A2 to spontaneous preterm birth. PMID: 16357110
  34. The promoter functionality of the two genomic regions of the hSVCT2 upstream of these alternative first exons in human Vascular Smooth Muscle Cells was tested. PMID: 16380174
  35. SVCT2 may switch between a number of states with characteristic properties, including an inactive conformation in the absence of Ca(2+)/Mg(2+) PMID: 17012227
  36. SVCT2 mRNA expression in human first-trimester chorionic villi but not in term placental tissue. PMID: 17092984
  37. SVCT2 expression can be regulated at the translational level by ascorbic acid and the redox state. PMID: 17291984
  38. SVCT1 is responsible for epidermal ascorbic acid supply, whereas SVCT2 mainly facilitates ascorbic acid transport in the dermal compartment PMID: 17664139
  39. The results suggest that uncharged His109 of hSVCT2, directly or indirectly, contributes to substrate binding through the hydrogen bond. PMID: 18247577
  40. all three proximal tubule segments expressed the transporter but the S3 segment had the highest expression; Ascorbic acid transport in these cells was regulated by a single kinetic component that depended on the sodium concentration, pH and temperature PMID: 18614995
  41. N-Glycosylation is therefore essential for SVCT2 functionality. PMID: 18619416
  42. estrogen receptor 1, vitamin C receptors SLC23A1 and SLC23A2, and matrix metalloproteinase MMP3 and MMP9 are associated with susceptibility to lymphoma PMID: 18636124
  43. For SLC23A2, overall, there was no colorectal adenoma association with haplotypes, but two SNPs located in intron 8 and exon 11 could be associated (odds ratio = 0.49, 95% confidence interval = 0.25-0.95 for haplotype G-C vs. haplotype C-C). PMID: 18791929
  44. hSVCT2 protein and mRNA are expressed at higher levels in HepG2 cells and native human liver, and the cloned hSVCT2 promoter has more activity in HepG2 cells. PMID: 18845575
  45. transports Vitamin C, a vital antioxidant, into the brain PMID: 19162177
  46. These results collectively suggested a default apical targeting of SVCT, which is consistent with the evolution-based prediction. PMID: 19216494
  47. increased expression during macrophage differentiation PMID: 19232538
  48. common variants in SLC23A2, a gene that directly regulates active transport of ascorbic acid, can impact gastric cancer risk PMID: 19243932
  49. SLC23A2 genetic variation alters HPV16-associated HNSCC while also highlighting the important role of citrus exposure in this disease. PMID: 19346260

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Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families Xanthine/uracil permease family, Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily
Tissue Specificity Ubiquitous.
Database Links

HGNC: 10973

OMIM: 603791

KEGG: hsa:9962

STRING: 9606.ENSP00000344322

UniGene: Hs.516866

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