Recombinant Human Surfeit locus protein 4 (SURF4)

Code CSB-CF022965HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
SURF4
Uniprot No.
Alternative Names
SURF4; SURF-4; Surfeit locus protein 4
Species
Homo sapiens (Human)
Expression Region
1-269
Target Protein Sequence
MGQNDLMGTAEDFADQFLRVTKQYLPHVARLCLISTFLEDGIRMWFQWSEQRDYIDTTWN CGYLLASSFVFLNLLGQLTGCVLVLSRNFVQYACFGLFGIIALQTIAYSILWDLKFLMRN LALGGGLLLLLAESRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFF SIVQNIVGTALMILVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFF QTMSVIGGLLLVVALGPGGVSMDEKKKEW
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

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 Q&A
Q:

We are solving topology of a membrane protein (5TM) now and want to know whether you have the capacity to express the protein. A pilot test will be performed first for an evaluation. We prefer to use baculovirus system in sf9 cells. Function is similar in yeast, human and all higher eukaryotes.
Target protein(s):
Attached is the sequence of the plasmid in the area of the Surf4 insert
Human sequence of Surf4 is NP_149351.1
We have fully functional Surf4 when we add a HA tag immediately after the starting methionine. I’d be hesitant to put anything longer there as this is one of those proteins where the amino terminus moves into the lumen of the ER without a leader sequence.
Unknown if functions as dimer or higher order but is found in dimers, trimers etc on western blots unless overnight incubation in LDS and 8M urea is used. We have binding assay but works to date only in native membrane preps, no binding yet when fully solubilized with n-Decyl-β-D-Maltopyranoside. May be in low affinity form when in DM or may not be folded correctly at all in this detergent.

Detailed plan is not necessary at this time, just a ball-park costs of various logical steps in your process. I have cDNA for human and yeast. If possible, could you please provide me an estimated cost and time for a pilot expression test of either/both of the proteins.

A:
Thanks for your inquiry,This is a 5-time transmembrane protein which has relatively strong hydrophobic. We recommend using our cell-free expression system. We have a very mature cell-free recombinant expression technique and have successfully expressed a variety of transmembrane proteins which are very different to express, even 10-time transmembrane proteins.
We have finished protein for human SURF4 and don't need to provide customize service. The details and distributor price are as below.
Code: CSB-CF022965HU
Name: Recombinant Human Surfeit locus protein 4(SURF4)
You can view the full information of sequence and tag on this page.
Uniprot Link: http://www.uniprot.org/uniprot/O15260
Lead Time: 25-35 working days

Target Background

Function
Endoplasmic reticulum cargo receptor that mediates the export of lipoproteins by recruiting cargos into COPII vesicles to facilitate their secretion. Acts as a cargo receptor for lipoproteins bearing both APOB and APOA1, thereby regulating lipoprotein delivery and the maintenance of lipid homeostasis. Synergizes with the GTPase SAR1B to mediate transport of circulating lipoproteins. Promotes the secretion of PCSK9. Also mediates the efficient secretion of erythropoietin (EPO). May also play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi.
Gene References into Functions
  1. SURF4 demonstrated aberrant amplification and increased expression in the tumor tissues of several human cancer patients. PMID: 29777698
  2. Silencing Surf4 together with ERGIC-53 or silencing the p24 family member p25 induced an identical phenotype characterized by a reduced number of ERGIC clusters and fragmentation of the Golgi apparatus without effect on anterograde transport. PMID: 18287528
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
Protein Families
SURF4 family
Database Links

HGNC: 11476

OMIM: 185660

KEGG: hsa:6836

STRING: 9606.ENSP00000361057

UniGene: Hs.512465

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