Recombinant Human coronavirus NL63 Non-structural protein 3 (3)

Code CSB-CF754294HIX
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
3; Non-structural protein 3; Protein 3; ns3; Accessory protein 3a
Human coronavirus NL63 (HCoV-NL63)
Expression Region
Target Protein Sequence
Protein Length
full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

To produce recombinant HCoV-NL63 Non-structural protein 3 protein, a well-established recombinant DNA technology is the key. A DNA template of Non-structural protein 3 was constructed with N-terminal 10xHis tag using the technique. Once the template was made, the recombinant HCoV-NL63 Non-structural protein 3 protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 1-225aa of the HCoV-NL63 Non-structural protein 3.

Human coronavirus NL63 non-structural protein 3 (Nsp3) is a viral protease involved in viral protein biogenesis, replication/transcription complex (RTC) formation, and regulation of host ubiquitinylation and ISGylation. Nsp3 exerts multiple roles in the viral life cycle. It can functions as a scaffold protein by interacting with itself and other proteins including viral Nsps and host proteins.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. The authors give evidence that ORF 3 protein is a structural N-glycosylated and virion-incorporated protein. PMID: 20078868
Subcellular Location
Host membrane; Multi-pass membrane protein.
Database Links

KEGG: vg:2943500

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