Recombinant Mouse ATP-sensitive inward rectifier potassium channel 8 (Kcnj8)

Code CSB-CF012060MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Kcnj8
Uniprot No.
Alternative Names
Kcnj8; ATP-sensitive inward rectifier potassium channel 8; Inward rectifier K(+ channel Kir6.1; Potassium channel, inwardly rectifying subfamily J member 8; uKATP-1
Species
Mus musculus (Mouse)
Expression Region
1-424
Target Protein Sequence
MLARKSIIPEEYVLARIAAENLRKPRIRDRLPKARFIAKSGACNLAHKNIREQGRFLQDI FTTLVDLKWRHTLVIFTMSFLCSWLLFAIMWWLVAFAHGDIYAYMEKGTMEKSGLESAVC VTNVRSFTSAFLFSIEVQVTIGFGGRMMTEECPLAITVLILQNIVGLIINAVMLGCIFMK TAQAHRRAETLIFSRHAVIAVRNGKLCFMFRVGDLRKSMIISASVRIQVVKKTTTPEGEV VPIHQQDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDISATDLANQDLEVIVILEGVV ETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVRVAAPRCSARELDE KPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRNNSIRRNNSSLMVPKVQFMTPEGNQC PSES
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Gene References into Functions
  1. Endotoxemia increases pulmonary Kir6.1 gene and protein expression. PMID: 29433570
  2. miR-20 may regulate myocardiac ischemia by targeting KATP subunit Kir6.1 to accelerate the cell apoptosis. PMID: 28786072
  3. S422L mutation in KCNJ8 gene is not a direct cause of J wave syndrome. PMID: 28928055
  4. Together, these results implicate the incorporation of native Kir6.1 subunits into pancreatic KATP channels and a contributory role for these subunits in the control of insulin secretion. PMID: 27956473
  5. data support the existence of an endothelial KATP channel that contains Kir6.1, is involved in vascular reactivity in the coronary circulation, and has a protective role in ischemia reperfusion. PMID: 28893911
  6. Similar to previous results in Kir6.1(-/-) myocytes, Kir6.1 gain of function myocytes demonstrate resistance (less volume derangement) to stress of cardioplegia. PMID: 27884343
  7. K(ATP) channel gain-of-function leads to increased myocardial L-type Ca(2+) current and contractility in Cantu syndrome. PMID: 27247394
  8. Kcnj8 mutation is responsible of heart conduction abnormalities in Cantu syndrome. PMID: 26142302
  9. our study demonstrated that exercise training up-regulated Kir6.1, improved tissue oxygenation recovery, and protected the heart against I/R injury PMID: 25474642
  10. Results suggest that the vascular KATP channel Kcnj8 is organ protective in diabetic conditions. PMID: 25825210
  11. The absence of Kir6.1 does not affect the contractile properties of myocytes during stress, suggesting the absence of Kir6.1 improves myocyte tolerance to stress via an unknown mechanism. PMID: 25872691
  12. Sulfhydration of SUR2B subunit modifies tyrosine nitration of Kir6.1 subunit within the KATP channel complex. PMID: 25552582
  13. Kir6.1 underlies the vascular smooth muscle KATP channel and has a key role in vascular reactivity and blood pressure control. PMID: 24914196
  14. Native K(ATP) channels in mouse vas deferens myocytes are a heterocomplex of K(IR)6.1 channels and SUR2B subunits. PMID: 24117345
  15. Kir6.1 knockdown exacerbates cerebral ischemia reperfusion-induced brain damage. PMID: 23663330
  16. A possible partnership between Cx43 and Kir6.1 will help in better understanding their roles in ischemia/hypoxia preconditioning. PMID: 22960107
  17. K(ATP) channel deregulation can have a critical impact on innate antiviral immunity in the heart. PMID: 21719711
  18. gene disruption causes prinzmetal angina in knockout mice PMID: 11984590
  19. Kir 6.1 gene expression was upregulated by almost 22-fold, whereas SUR2B was downregulated by threefold after inflammation. Decreased motility of colon during inflammation may be associated with changes in transcriptional regulation of Kir 6.1 and SUR2B. PMID: 14962845
  20. Kir6.1-containing K(ATP) channel, by coupling vasoreactivity with metabolic demand, provides a vital feedback element for cardiovascular tolerance in endotoxic shock. PMID: 17077304
  21. Expression of functional Kir6.1 channels regulates glutamate release at CA3 synapses in generation of epileptic form of seizures. PMID: 17883401
  22. Study identified a profound susceptibility to infection phenotype due to a null allele of Kcnj8, encoding Kir6.1, a protein that combines with SUR2 to form an ATP-sensitive potassium channel (K(ATP)) expressed in coronary artery. PMID: 18026101
  23. The data suggest it is premature to assign Kir6.1 a role in mitoK(ATP) on the basis of immunoreactivity alone. PMID: 18068667
  24. SUR2 and Kir6.1 mRNAs were found to be the most abundant in ventricular fibroblasts. PMID: 19166858

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ8 subfamily
Database Links
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