Recombinant Mouse Cadherin-11 (Cdh11)

Code CSB-CF005036MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Cdh11
Uniprot No.
Alternative Names
Cdh11; Cad-11; Cadherin-11; OSF-4; Osteoblast cadherin; OB-cadherin
Species
Mus musculus (Mouse)
Expression Region
54-796
Target Protein Sequence
GWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHEIYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVTITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGIELFEITTDYETQDGVVKLKKPVDFETKRAYSLKIEAANVHIDPKFISNGPFKDTVTVKISVEDADEPPMFLAPSYIHEVQENAAAGTVVGRVHAKDPDAANSPIRYSIDRHTDLDRFFTINPEDGFIKTTKPLDREETAWLNISVFAAEIHNRHQETKVPVAIRVLDVNDNAPKFAAPYEGFICESDHPKALSNQPIVTVSADDQDDTANGPRFIFSLPPEIMHNPNFTVRDNRDNTAGVYARRGGFSRQKQDFYLLPIVISDGGIPPMSSTNTLTIKVCGCDVNGALLSCNAEAYILNAGLSTGALIAILACIVILLVIVVLFVTLRRQKKEPLIVFEEEDVRENIITYDDEGGGEEDTEAFDIATLQNPDGINGFIPRKDIKPEYQYMPRPGLRPAPNSVDVDDFINTRIQEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESATTDSDLDYDYLQNWGPRFKKLADLYGSKDTFDDDS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Gene References into Functions
  1. Expression of cadherin-11, an adhesion molecule known to promote cell migration and compaction, was upregulated by intermediate-dose BMP7. BMP7-induced cadherin-11 expression was inhibited by cotreatment with SB203580 and PD98059. PMID: 28867673
  2. data demonstrate an important role for CDH11 in the development of skin fibrosis in Tsk-1 mice. These data add to the growing evidence for the important role of CDH11 in tissue fibrosis and fibrotic disease such as systemic sclerosis PMID: 29112946
  3. stromal fibroblasts expressing cadherin-11 regulate adipose tissue inflammation PMID: 28758901
  4. engagement of CDH11 through homophilic interactions, led to swift activation of the TGF-beta and ROCK pathways as evidenced by phosphorylation of downstream effectors. PMID: 27311482
  5. Loss of CDH11 gene is associated with retinoblastoma. PMID: 27750399
  6. Overexpression of Cad-11 upregulates RhoA and Sox9 to induce calcification and extracellular matrix remodeling in adult aortic valves. PMID: 27312222
  7. Cad-11 depleted cells in culture contained few filopodia, stress fibers, or contact inhibited locomotion PMID: 26188246
  8. rFN/CDHs may induce hMSCs differentiation into osteoblasts and PLGA/collagen nanofibers loaded with rFN/CDHs have potential in bone tissue engineering. PMID: 24844413
  9. Cadherin-11 regulates the expression of TGF-B1 and affects smooth muscle cell differentiation through a pathway that was dependent on TGF-B receptor II. PMID: 24741067
  10. Cadherin-11 may play a role in renal cell carcinoma bone metastasis. PMID: 24587095
  11. Data indicate that Cadherin-11 (CDH11) is increased in cells during normal cerebral cortical development. PMID: 23951053
  12. We also demonstrated that CDH11 acts as an inhibitor of tumour growth, motility and dissemination. PMID: 22374749
  13. CDH11-deficient mice had decreased fibrotic endpoints in the bleomycin model of pulmonary fibrosis compared to wild-type mice. PMID: 21990376
  14. The pattern of haplotype diversity across the quantitative trait loci (QTL) region and expression profiles of QTL genes suggested that of the 12, cadherin 11 (Cdh11) was most likely the causal gene. PMID: 21638317
  15. cad-11 modulates synovial fibroblasts to evoke inflammatory factors that may contribute to the inflammatory process in RA. PMID: 21536877
  16. Cdh2 and Cdh11 are crucial regulators of postnatal skeletal growth and bone mass maintenance, serving overlapping, yet distinct, functions in the osteogenic lineage. PMID: 20605916
  17. Cdh11 acts as a tumor suppressor in a murine retinoblastoma model by facilitating tumor cell death PMID: 20421947
  18. Cadherin 11 expression initially appears in motor columns before extending to the whole spinal cord, dorsal root ganglia, and sensory-motor peripheral nerves; later, its expression decreases in nervous tissues and becomes predominant in mesenchymes. PMID: 12139922
  19. Cdh11 in intercellular junctions interconnect dermal papilla cells in developing hair follicles and seem to be responsible for establishment of these junctions. PMID: 12524684
  20. A 2.62 Mbp minimal region of genomic loss of chromosome 16q22 was found in retinoblastoma which contains a hotspot CDH11, implicating CDH11as a potential tumor suppressor gene in retinoblastoma. PMID: 15383628
  21. cadherin-11 directly regulates the differentiation of mesenchymal cells into the cells of the osteo-lineage and the chondro-lineage in a different manner from N-cadherin PMID: 15476585
  22. Cadherin-11 enhanced axon growth and prevented interactions occurring between growing axons, providing evidence for a role of cadherin-11 in the control of growth cone progression. PMID: 15797718
  23. Together, these studies implicate cadherin-11 in synovial tissue and lining layer formation and provide an in vitro system to model fibroblast-like synoviocyte behavior and function in organizing the synovial tissue. PMID: 16651616
  24. synovial cadherin-11 determines the behavior of synovial cells in their proinflammatory and destructive tissue response in inflammatory arthritis PMID: 17255475
  25. Results show that tendon formation in the developing limb requires precise regulation of cell shape via cadherin-11-mediated cell-cell junctions and coaxial alignment of plasma membrane channels in longitudinally stacked cells. PMID: 17562872
  26. the results show a link between Trio activity,Rac1 activation,neuronal organization,and recruitment of Cadherin-11 receptor to form a complex with Trio in hindbrain embryology. PMID: 17898204
  27. fibroblast-like synoviocytes express both N-cadherin and cadherin 11, but they do not coimmunoprecipitate, suggesting these cadherins are not in the same molecular complex PMID: 18383368

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Selectively expressed in osteoblastic cell lines, precursor cell lines of osteoblasts, and primary osteoblastic cells from calvaria, as well as in lung, testis, and brain tissues at low levels.
Database Links
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