Recombinant Mouse Meprin A subunit alpha (Mep1a)

Code CSB-CF013697MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Mep1a
Uniprot No.
Alternative Names
Mep1a; Meprin A subunit alpha; Endopeptidase-2; MEP-1
Species
Mus musculus (Mouse)
Expression Region
65-747
Target Protein Sequence
NAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARSTMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCIAGLIFLTFVTFSTTNGKLRQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. no significant dentin malformation was observed in Mep1b (-/-) or Mep1a (-/-) deficient mice. PMID: 27628095
  2. meprin alpha and meprin beta join the modulators of Reelin signalling as they cleave Reelin at a specific site and are upregulated under specific pathological conditions. PMID: 26491063
  3. While meprin A only cleaved protein kinase A (PKA) catalytic subunit beta1, meprin B cleaved all three PKA catalytic isoforms. PMID: 25354939
  4. meprin alpha and meprin beta are unique in their ability to process and release both C- and N-propeptides from type I procollagen in vitro and in vivo PMID: 23940311
  5. Absence of meprin A aggravates chronic inflammation and lack of meprin B affords some protection from injury. Manipulation of expression of meprin gene products may have therapeutic potential. PMID: 21071511
  6. conserved sequences essential for zinc binding and enzymatic activity were determined by site-directed mutagenesis PMID: 12437102
  7. meprin-alpha may play a role in the pathogenesis of diabetic nephropathy and the benefits of ACE inhibitor therapy PMID: 15942051
  8. determined the sites of glycan attachment and assessed their ability to affect the formation and stability of the homo-oligomer PMID: 17040911
  9. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury. PMID: 17377510
  10. meprin A, a multimeric metalloprotease expressed in the brush borders of kidney proximal tubules initially truncates mouse BNP in the N terminus to mBNP7-32 PMID: 17823376
  11. Meprin A contributes to the renal and urogenital pathogenesis of endotoxicity. PMID: 18971209
  12. meprin alpha/beta null (alpha(-/-)/beta(-/-)) mice had decreased prevalence of resident monocytes and natural killer (NK) cells in blood, with a concomitant accumulation of inflammatory monocytes and NK cells in bone marrow. PMID: 19110362
  13. MEP1A is a UC susceptibility gene and indicate that decreased meprin-alpha expression is associated with intestinal inflammation in IBD patients and in a mouse experimental model of IBD. PMID: 19262505

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Peptidase M12A family
Tissue Specificity
Kidney, intestinal brush borders and salivary ducts.
Database Links
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