Product Details

Target Names

Otop1

Uniprot No.

Q80VM9

Research Area

Transport

Alternative Names

Otop1; Proton channel OTOP1; Otopetrin-1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

1-600aa

Target Protein Sequence

MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASGPRRGASVPQKLAETLSSQYGLNVFVAGLLFLLAWAVHATGVGKSDLLCVLTALMLLQLLWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAFITVVLGCLKVAYFIGFSECLSATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLLLWANSVLNESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQILASTMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHIGRSKSKSESALIMFYLYAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLLVATGSGSWLLSWGSILAIACAETRPPYTWYNLPYSVLVIVEKYVQNIFIIESVHLEPEGVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAVNGNLCLQQRCGKEDQESGWEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Tag Info

C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it’s possible to develop.

Form

Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.

Buffer

Lyophilized from PBS, 6% Trehalose, pH 7.4

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Proton-selective channel that specifically transports protons into cells. Proton channel activity is only weakly-sensitive to voltage. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes. In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration. Probably acts by maintaining the pH appropriate for formation of otoconia. Regulates purinergic control of intracellular calcium in vestibular supporting cells. May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol. Also involved in energy metabolism, by reducing adipose tissue inflammation and protecting from obesity-induced metabolic dysfunction.

Gene References into Functions

Otop1 defines a unique target of cytokine signaling that attenuates obesity-induced adipose tissue inflammation and plays an adaptive role in maintaining metabolic homeostasis in obesity. PMID: 24379350
Data show that tlt and mlh mutations uncouple Otopetrin 1 from inhibition of P2Y receptor function. PMID: 21236346
OTOP1 is expressed at the apex of supporting cells. It increases cytosolic Ca in response to purinergic agonists by blocking mobilization of Ca from intracellular stores in an extracellular Ca-dependent manner & by mediating extracellular Ca influx. PMID: 20554841
a non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice is caused by mutations in otopetrin 1 PMID: 12651873
Otopetrin 1 encodes a multi-transmembrane domain protein expressed in the developing otocyst. Mouse mutants tilted and mergulhador carry recessive mutations in Otop1 that result in otoconial agenesis without causing collateral deafness. PMID: 12651873

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Otopetrin family

Tissue Specificity

Detected in embryonic inner ear macular epithelia. Expressed in thymus, heart, kidney, skin, vestibular system of the inner ear, sour taste cells, brown adipose tissue, heart, uterus, dorsal root ganglion, adrenal gland, lactating mammary gland and stimul

Database Links

KEGG: mmu:21906

STRING: 10090.ENSMUSP00000109734

UniGene: Mm.204765