Recombinant Mouse Potassium-transporting ATPase subunit beta (Atp4b)

Code CSB-CF002343MO
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Source in vitro E.coli expression system
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Product Details

Target Names
Atp4b
Uniprot No.
Alternative Names
Atp4b; Potassium-transporting ATPase subunit beta; Gastric H(+/K(+ ATPase subunit beta; Proton pump beta chain
Species
Mus musculus (Mouse)
Expression Region
1-294
Target Protein Sequence
MAALQEKKSCSQRMAEFRHYCWNPDTGQMLGRTPARWVWISLYYAGFYVVMTGLFALCIYVLMQTIDPYTPDYQDQLKSPGVTLRPDVYGERGLKISYNVSENSSWAGLTHTLHSFLAGYTPASQQDSINCTSEKYFFQESFAAPNHTKFSCKFTADMLQNCSGLADPSFGFEEGKPCFIIKMNRIVKFLPSNNTAPRVDCTFQDDPQKPRKDTEPLQVEYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNVPKNMQVSIVCKILADHVTFNNPHDPYEGKVEFKLTIQK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.
Gene References into Functions
  1. H(+) /K(+) ATPase beta subunit knockout mice had decreased bone mineral content and bone mineral density, reduced cortical thickness and inferior mechanical bone strength. PMID: 21882222
  2. The endogenous H+/K+ ATPase beta autoantigen in normal mice plays a critical role in the the tolerance of pathogenic H/Kbeta-specific T cells. PMID: 12193702
  3. Contributes to the unique ultrastructure of secretory membranes in gastric parietal cells. PMID: 12195293
  4. These results may imply that the H(+),K(+)-ATPase in the cochlear lateral wall is crucial for K(+) circulation and thus plays a critical role in generation of endocochlear potential. PMID: 16822945
Subcellular Location
Apical cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.
Protein Families
X(+)/potassium ATPases subunit beta family
Tissue Specificity
Expressed in parietal cells (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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