Recombinant Mouse Pre T-cell antigen receptor alpha (Ptcra)

Code CSB-CF018961MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Ptcra
Uniprot No.
Alternative Names
Ptcra; Pre T-cell antigen receptor alpha; pT-alpha; pTa; gp33; pT-alpha-TCR
Species
Mus musculus (Mouse)
Expression Region
17-199
Target Protein Sequence
LPSGIAGTPFPSLAPPITLLVDGRQHMLVVCLVLDAAPPGLDNPVWFSAGNGSALDAFTYGPSLAPDGTWTSLAQLSLPSEELEAWEPLVCHTRPGAGGQNRSTHPLQLSGESSTARSCFPEPLGGTQRQVLWLSLLRLLLFKLLLLDVLLTCSHLRLHVLAGQHLQPPPSRKSLPPTHRIWT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. Isoform 1 acts to retain most TCRB intracellularly, while isoform 2 permits higher levels of cell surface TCRB expression and facilitates signaling from the CD3-TCRB complex.
Gene References into Functions
  1. pre-TCR expression is not essential for thymic differentiation to DP cells or for V-DJbeta suppression PMID: 21673984
  2. Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis PMID: 11891328
  3. The cytoplasmic tail of pTalpha plays a critical role in T lymphocyte development. PMID: 11927911
  4. pTalpha plays a role in protecting developing thymocytes from DNA damage in mice PMID: 12114514
  5. the dosage of E2A, HEB, and SCL determines pT alpha gene expression in immature T cells PMID: 12566462
  6. when in competition with pTalpha, TCRalpha induced defective proliferation, survival, and differentiation of alphabeta T lymphocyte precursors PMID: 14993248
  7. expression is up-regulated in regulatory T cells PMID: 19461123

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Isoform 1 is expressed at higher levels than isoform 2 in the thymus while only isoform 2 is expressed in polyclonal beta-only cells. Isoform 1 shows a predominant expression in immature thymocytes.
Database Links

KEGG: mmu:19208

UniGene: Mm.215173

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