Recombinant Mouse Protein wntless homolog (Wls)

Code CSB-CF735316MO
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
Wls; Gpr177; Protein wntless homolog; Integral membrane protein GPR177; Protein evenness interrupted homolog; EVI
Mus musculus (Mouse)
Expression Region
Target Protein Sequence
Protein Length
Full Length of Mature Protein
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
Gene References into Functions
  1. WLS role in the principal cells of the caput epididymidis during sperm maturation PMID: 29152689
  2. Wntless-deficient macrophages presented a unique subset of M2-like macrophages with anti-inflammatory, reparative, and angiogenic properties. Serial echocardiography studies revealed that mice lacking macrophage Wnt secretion showed improved function and less remodeling 30 days after myocardial infarction. PMID: 28062479
  3. GPR177 in Sertoli cells had no apparent influence on spermatogenesis, whereas loss of GPR177 in germ cells disrupted spermatogenesis in an age-dependent manner. PMID: 27362799
  4. these results suggest that Gpr177 controls epithelial initiation of the fungiform placode through signaling via epithelial Wnt ligands. PMID: 24736288
  5. regulates chondrogenesis and osteogenesis through both canonical and noncanonical Wnt signaling PMID: 26249818
  6. Ablation of Wntless in endosteal niches impairs lymphopoiesis rather than hematopoietic stem cells maintenance. PMID: 26173091
  7. Wls-mediated secretion of Wnt ligands from the developing ventral body wall mesenchyme plays a critical role in fusion of the sternum and closure of the secondary body wall. PMID: 25727890
  8. eletion of the Wls gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation and root elongation. PMID: 25595365
  9. The existence of spatial compartmentation in the rhombic lip and the interplay between Wls, Math1, and Pax6 in the rhombic lip provides novel views of early cerebellar development. PMID: 25209290
  10. Retromer dependent recycling of the Wnt secretion factor Wls is dispensable for stem cell maintenance in the mammalian intestinal epithelium PMID: 24130821
  11. The loss of Wls in the ventral epithelium, which blocks the secretion of Wnt proteins, resulted in dysgenesis of ventral musculature and genitourinary tract during embryonic development. PMID: 24394376
  12. Gpr177 regulates pulmonary vasculature development. PMID: 23884445
  13. Evi expression in psoriatic skin biopsies is down-regulated, suggesting that Evi-deficient mice developed skin lesions that resemble human psoriasis. PMID: 23918954
  14. Wnt production mediated by Gpr177 is essential for mammary morphogenesis. PMID: 23457599
  15. Wntless is necessary for respiratory epithelial cell differentiation. PMID: 23523683
  16. Here we report that Wnt production mediated by Gpr177, the mouse Wls ortholog, is essential for hair follicle induction. PMID: 23190887
  17. Wls may regulate hair follicle induction by mediating the Wnt/beta-catenin pathway. PMID: 23029304
  18. Wls-deficient osteoblasts had a defect in differentiation and mineralization, with significant reductions in the expression of key osteoblast differentiation regulators. PMID: 22745162
  19. Wntless plays a role in signaling regulation during the formation of cancer in addition to its role as a retromer protein in mammalian systems. PMID: 22726896
  20. These findings provide a comprehensive view of the Wls-mediated role of Wnts in limb patterning and tissue morphogenesis. PMID: 22377357
  21. Data demonstrate the importance of Gpr177 in Wnt1-mediated development of the mouse embryo, suggesting an overlapping function of Wnts 1 and 3a. PMID: 21246653
  22. The relatively ubiquitous expression of GPR177 suggests that this protein may serve to regulate Wnt secretion in a variety of embryonic and adult tissue types. PMID: 20652957
  23. Findings suggest that the Gpr177-mediated regulation of Wnt is crucial for organogenesis in health and disease. PMID: 20549736
  24. These findings suggest a previously unrecognized role for GPR177 in regulating cellular response to opioid agonist drugs, and could create new avenues of investigation regarding the pharmacotherapy of drug dependence. PMID: 20214800
  25. A reciprocal regulation of Wnt and Gpr177 is essential for the patterning of the anterior-posterior axis during mammalian development. PMID: 19841259

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Subcellular Location
Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein.
Protein Families
Wntless family
Tissue Specificity
Expressed in the brain, skeletal muscle, heart muscle, lung, gut, liver, and kidney (at protein level). In the brain, expressed in the cortex, striatum, hippocampus and to a lesser extent in the cerebellum (at protein level). Expressed in kidney, lung, sk
Database Links
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