Product Details

Target Names

Slc14a1

Uniprot No.

Q8VHL0

Alternative Names

Slc14a1Urea transporter 1; Solute carrier family 14 member 1; Urea transporter B; UT-B; Urea transporter; erythrocyte

Species

Mus musculus (Mouse)

Source

in vitro E.coli expression system

Expression Region

1-384

Target Protein Sequence

MEDSPTMVKVDRGENQILSCRGRRCGFKVLGYVTGDMKEFANWLKDKPVVLQFMDWILRGISQVVFVSNPISGILILVGLLVQNPWWALCGCVGTVVSTLTALLLSQDRSAIAAGLQGYNATLVGILMAVFSNKGDYFWWLIFPVSAMSMTCPVFSSALSSVLSKWDLPVFTLPFNMALSMYLSATGHYNTFFPSKLFTPVSSVPNITWSELSALELLKSLPVGVGQIYGCDNPWTGGIFLCAILLSSPLMCLHAAIGSLLGVIAGLSLAAPFEDIYFGLWGFNSSLACIAIGGMFMALTWQTHLLALACALFTAYFGACMAHLMAVVHLPACTWSFCLATLLFLLLTTKNPNIYRMPLSKVTYSEENRIFYLQNKKRMVESPL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Protein Length

Full Length

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.

Gene References into Functions

functional deficiency of all UTs caused a urea-selective urine-concentrating defect with little physiological abnormality in extrarenal organs. PMID: 27914708
We conclude that transgenic expression of UT-A1 restores basal urea permeability to the level in wild-type mice but does not restore vasopressin-stimulated levels of urea permeability. This information suggests that transgenic expression of UT-A1 alone in mice lacking UT-A1 and UT-A3 is sufficient to restore urine-concentrating ability PMID: 26407594
These results demonstrate that the descending thin limb (DTL) of the intermediate loop is composed of type I epithelium and expresses both UT-A2. The functional role of the DTL of the intermediate loop may be distinct from the short or long loops. PMID: 27091563
Studies indicate that expression of urea transporter UT-B confers high urea permeability to erythrocytes. PMID: 25298342
Studies indicate that urea transporter Slc14a1 UT-B is an indispensable transporter involved in maintaining physiological functions in different tissues. PMID: 25298344
Data suggest that urea transporter UT-B may play an important role in protecting bladder urothelium by balancing intracellular urea concentration. PMID: 24204711
Thienoquinoline PU-14 is a selective UT inhibitor and has urea-selective diuretic activity. PMID: 23486518
AQP9 and unidentified UT-A urea channels constitute primary but redundant urea facilitators in murine hepatocytes PMID: 23042941
Urea transporter-B-null mice showed defective urine concentrating ability. PMID: 22289137
UT-B null mice exhibited depression-like behavior, with urea accumulation, nitric oxide reduction, and selective neuronal nitric oxide synthase level increase in hippocampus. PMID: 21750947
UT-A2 deletion in UT-B knockout mice partially remedies the urine concentrating defect caused by UT-B deletion. PMID: 21849488
UT-B has a role in urinary concentrating ability in mice PMID: 12133842
potassium depletion is associated with reduced expression of UT-A1, UT-A3, and UT-B but increased expression of UT-A2 PMID: 12952854
UT-B-deficient mice reveal that countercurrent exchange of urea in renal medullary vessels and red blood cells accounts for a major part of the kidney's concentrating ability and for the adaptation of renal urea handling during a high-protein intake. PMID: 12965892
UT-B null mice have a normal glomerular filtration rate but moderately reduced urea clearance PMID: 15821253
This review focuses on the erythrocyte urea transporter UT-B. PMID: 17264984
UT-B plays an important role in eliminating urea produced by Sertoli cells and UT-B deletion causes both urea accumulation in the testis and early maturation of the male reproductive system. PMID: 17475664
Data indicate that UT-B deletion caused the dynamic expression regulation of TNNT2 and ANP, and these proteins may provide new clues to investigate the molecular events involved in cardiac conduction. PMID: 19132680
Results provide the first evidence that UT-B deletion causes progressive heart block in mice. PMID: 19471871

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Note=Restricted to the basolateral membrane in various portions of the urothelium.

Protein Families

Urea transporter family

Tissue Specificity

Expressed in brain, kidney, heart, liver, lung, skeletal muscle, spleen, testis, ureter and urinary bladder (at protein level). Along the gastrointestinal tract, detected in colon, jejunum and stomach (at protein level). In the kidney, expressed in some m

Database Links

KEGG: mmu:108052

STRING: 10090.ENSMUSP00000125114

UniGene: Mm.33832