Recombinant Mouse Zinc transporter 8 (Slc30a8) is produced through an in vitro E.coli expression system and includes the complete protein spanning amino acids 1 to 367. The protein features an N-terminal 10xHis tag that simplifies purification and detection processes. SDS-PAGE analysis confirms the product achieves purity levels above 85%, which appears to provide reliable performance for research work.
Zinc transporter 8 (Slc30a8) represents a key protein in regulating zinc ion movement across cellular membranes. Its role in maintaining zinc homeostasis seems critical for numerous cellular functions. The protein shows particular importance in pancreatic beta cells, where it likely contributes to insulin secretion and glucose metabolism. This makes it an increasingly important target for metabolic research.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Antibody Development and Validation
This full-length recombinant mouse Slc30a8 protein may serve as an effective immunogen for creating polyclonal or monoclonal antibodies targeting zinc transporter 8. The N-terminal 10xHis tag makes purification and immobilization straightforward for antibody screening assays. Researchers can apply this protein in ELISA-based screening to find high-affinity antibodies and confirm their specificity using Western blot analysis. The >85% purity level appears adequate for immunization protocols and follow-up antibody characterization work.
2. Protein-Protein Interaction Studies
His-tagged Slc30a8 protein can be applied in pull-down assays to discover potential binding partners or regulatory proteins that interact with zinc transporter 8. The 10xHis tag permits efficient attachment to nickel-affinity matrices, allowing researchers to capture interacting proteins from cell lysates or purified protein collections. This strategy might help reveal the molecular mechanisms behind Slc30a8 function and regulation within zinc homeostasis pathways.
3. Biochemical Characterization and Binding Studies
This recombinant protein offers researchers a useful tool for examining Slc30a8's biochemical properties. Studies could include zinc binding affinity and specificity analysis using methods like isothermal titration calorimetry or fluorescence-based assays. The full-length design preserves the complete protein structure, making it suitable for thorough biochemical evaluation. Scientists can also test the protein's stability across different buffer conditions and temperature ranges to refine experimental approaches.
4. Comparative Species Analysis
Mouse Slc30a8 protein can be applied in comparative research with human or other mammalian zinc transporter 8 counterparts to study evolutionary conservation and species-specific variations. Cross-reactivity experiments with antibodies developed against human Slc30a8 may reveal conserved binding sites and functional regions. This application appears particularly useful for researchers conducting translational studies where mouse models help explain human zinc transporter biology.
