Recombinant Probable muscarinic acetylcholine receptor gar-2 (gar-2)

Code CSB-CF600999CXY
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
gar-2
Uniprot No.
Alternative Names
gar-2; F47D12.1; Muscarinic acetylcholine receptor gar-2; G-protein-linked acetylcholine receptor 2
Species
Caenorhabditis elegans
Expression Region
1-627
Target Protein Sequence
MAVASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIP VYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTP GRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYW TTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEMRRPEVSVRTSDAGNSSSDSPNDT SNSSKCFRTAPPTTTVQTTQTNVGTPPPVFRNHMTLHNNNMDFTKDNEIVRPPTPPDDNT YSNPNFSMISEQLTNGFSRQEPSSVIERESTAPCVSPEPSHASLENEFNENHHAHFKPEL SLPFIDADSVSSMVGHDDLRRAMSIRISRSVSMQGTARATPVIEIVENLEEALKICENLE ELREDENKNEEEKQKNGLENGGMNHVIIANDEQQPSTSKESEQKEEMTPENHDPNEVKVP LIAVSRVESVKSTAGGKVRRLITQMRSHSIRSKRKANKNKSVLSALNFFQRKKEYKSRSE NRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPL NPFCYAMANQQFKKTLTRIFKGDFRRV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Regulates the activity of ventral cord motor neurons. Couples to the G(o) alpha G-protein subunit goa-1 to negatively regulate cholinergic receptor activity in the presence of high levels of the neurotransmitter acetylcholine in ventral cord motor neurons. As acetylcholine depolarizes body wall muscles, modulation of acetylcholine levels most likely results in the control locomotory behavior and egg-laying.
Gene References into Functions
  1. This study identify a muscarinic receptor (GAR-2) and the GABA(B) receptor dimer (GBB-1/2) that detect synaptically released ACh and GABA and regulate the locomotion. PMID: 18614679
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell projection, axon.
Protein Families
G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily
Tissue Specificity
Expressed in putative sensory neurons, many cells of the ventral cord and in the HSN motor neurons. Expressed in some cholinergic motor neurons and GABAergic motor neurons, which are the two major types of ventral cord motor neurons.
Database Links

UniGene: Cel.8076

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