Recombinant Rabbit G-protein coupled bile acid receptor 1 (GPBAR1)

Code CSB-CF768106RB
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
GPBAR1
Uniprot No.
Alternative Names
GPBAR1; TGR5; G-protein coupled bile acid receptor 1
Species
Oryctolagus cuniculus (Rabbit)
Expression Region
1-330
Target Protein Sequence
MTPNSTGEVPGPIPRGALELSLALASLIIAANLLLALGIACDRRLRSPPAGCFFLSLLLA GLLTGLALPTLPGLWRQSHRGYWSCLLVYLAPNFSFLSLLANLLLVHGERYVAVLRPLQP PGSIRLALLLTWTGPLLFASLPALGWNHWGPEANCSSQTIFPAPYLYLEVYGLLLPAVGA AALLSAHVLLAAHRQLQDIRRLERAVCRDAPSALARALTWRQARAQAGATLLFGLCWGPY VATLFLSVLAYEQRPPLGPGTLLSLLSLGSASAAAVPVAMGLGDHRYTAPWRAAARRWLR GLRGRGSQASPGPSTAYHTSSQSSVDVDLN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for bile acid. Bile-acid binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids. Involved in bile acid promoted GLP1R secretion.
Gene References into Functions
  1. TGR5 has been linked to signaling pathways involved in metabolism, cell survival, proliferation and apoptosis, which suggest a possible role of TGR5 in cancer development. PMID: 23782454
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Expressed at high level in spleen. Expressed at lower level in thymus, heart, lung, liver, kidney, ileum, blood and adherent alveolar macrophage cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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