Recombinant Rat 5-hydroxytryptamine receptor 1B(Htr1b)

Code CSB-CF010882RA
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names Htr1b
Uniprot No. P28564
Alternative Names Htr1b; 5ht1b5-hydroxytryptamine receptor 1B; 5-HT-1B; 5-HT1B; Serotonin receptor 1B
Species Rattus norvegicus (Rat)
Expression Region 1-386
Target Protein Sequence MEEQGIQCAPPPPATSQTGVPLANLSHNCSADDYIYQDSIALPWKVLLVALLALITLATT LSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDF WLSSDITCCTASIMHLCVIALDRYWAITDAVDYSAKRTPKRAAIMIVLVWVFSISISLPP FFWRQAKAEEEVLDCFVNTDHVLYTVYSTVGAFYLPTLLLIALYGRIYVEARSRILKQTP NKTGKRLTRAQLITDSPGSTSSVTSINSRVPEVPSESGSPVYVNQVKVRVSDALLEKKKL MAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHMAIFDFFNWLGYLNSLI NPIIYTMSNEDFKQAFHKLIRFKCTG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.
Gene References into Functions
  1. findings indicate that stimulation of the 5-HT1B receptor can enhance methylphenidate-induced gene regulation. PMID: 28720013
  2. in rats immunized with M4R and M3R peptides the sensitivity of ACSS thyrocytes to TSH were decreased, whereas in rats iimunized with 5-HT1BR peptide the deficit of thyroid hormones was associated with decreased level of TSH PMID: 26547948
  3. Results show that TRPM8 and the 5-HT1BR interact directly in a novel signalling complex that incorporates PLD1, which mediates the facilitatory influence by means of its product phosphatidic acid acting on PIP 5-kinase to increase production of PIP2 PMID: 24269608
  4. Adaptive changes on 5-HT1B receptors induced by chronic venlafaxine administration exhibit regional brain differences, with the hypothalamus the site of drug action. PMID: 23609771
  5. Single artery occlusion without significant visible infarct resulted in locally increased ETB, angiotensin type 1 and 5-hydroxytryptamine 1B receptor-mediated contractile responses only in segments located downstream of the occlusion site. PMID: 23988741
  6. 5-HTB/D receptors have a role in the reduction of formalin-induced nociception and secondary allodynia/hyperalgesia. produced by antimigraine drugs in rats PMID: 23583574
  7. Secondhand smoke exposure upregulates cerebrovascular 5-HT(1) (B) receptors in rats associated with Raf/ERK/MAPK activation. PMID: 22883081
  8. NXN-188 inhibits CGRP release from migraine-relevant cephalic tissues. PMID: 23155193
  9. 5-HT may have a minor physiological role in blood flow regulation via 5-HT1B receptor activation in the smooth muscle of the rat middle cerebral artery. PMID: 22659115
  10. expressional upregulation of vasoconstrictor receptors in cerebral arteries in global ischemic stroke PMID: 22848635
  11. ET(B) receptor-mediated contraction was mediated by calcium from intracellular and extracellular sources, whereas 5-HT(1B) receptor-mediated contraction was solely dependent on extracellular calcium. PMID: 22585122
  12. Data show that during epileptogenic periods following hypoxic-ischemia, both 5-HT(1A) and 5HT(1B) receptor immunoreactivities were decreased within the lesioned and the non-lesioned hippocampus. PMID: 21681557
  13. we demonstrate higher constitutive activation and spontaneous endocytosis for the axonal 5-HT(1B) R. PMID: 21801291
  14. 5-HT1b receptors modulate the long polysynaptic excitatory postsynaptic potentials in motoneurons, which adapt to a loss of serotonin after chronic spinal transection in rats. PMID: 21653728
  15. Bilateral administration of 5HT1B receptor agonist, CGS-12066A into the medial prefrontal cortex decreased maze exploration indicating an anxiogenic response. SB-224289 reversed this, indicating a role for 5HT1B in anxiety behavior. PMID: 21982809
  16. Neither acute social defeat stress , nor acute tailshock stress had a significant impact on 5-HT(1B) mRNA expression in the striatum PMID: 21718722
  17. Dtta show that exposure to magnetic fields below the current occupational limit does not affect the physiological function involved in 5-HT(1B) receptor subtypes. PMID: 21047499
  18. activation of 5-HT1B receptors in the ventral-orbital prefrontal cortex has anti-aggressive effects in postpartum female rats PMID: 21107539
  19. results indicate that 5-hydroxytryptamine (serotonin) receptor 1B receptors expressed on nucleus accumbens shell projection neurons facilitate ethanol drinking PMID: 20843634
  20. 5-HT(1B), 5-HT(1D)and 5-HT(1F) receptor expression are equally represented in trigeminal ganglia and the dorsal root ganglia. PMID: 20833155
  21. vestibular ganglion cells were immunoreactive for 5-HT(1B) receptors in macaques and rats PMID: 20510890
  22. Activation of 5-HT1B receptors inhibits the vasodepressor sensory CGRPergic outflow in pithed rats. PMID: 20385119
  23. differential expression of 5-HT1AR and 5-HT1BR in central nucleus of the amygdala neurones projecting to the caudal dorsomedial medulla suggests that these neurones may act differentially in controlling autonomic expression of emotional behavior PMID: 20226766
  24. shows that the activity of bed nucleus of the stria terminalis neurons can be indirectly modulated by 5-HT by 5-HT1B receptors, and suggests a potential new target for the development of novel treatments for depression and anxiety disorders PMID: 19963045
  25. 5-HT release in SNr is not devoid of autoreceptor regulation by endogenous 5-HT, but rather is under modest control which only weakly limits 5-HT signaling. PMID: 19819310
  26. Expression of 5-HT1B receptor mRNA is decreased in the cerebellum and frontal cortex following bilateral adrenalectomy; 5-HT1B receptor mRNA levels are unchanged in hippocampus and striatum in adrenalectomized animals. PMID: 12668254
  27. The release of 5-HT after subarachnoid hemorrhage activates 5-HT1B receptors and the synthesis of 20-HETE contributing to the acute fall in rCBF by potentiating the vasoconstrictor response of cerebral vessels to 5-HT. PMID: 12677022
  28. 5-HT1B receptor stimulation and blockade result in opposite effects in two types of cognitive tasks in the rat PMID: 12838273
  29. Elevated dorsal raphe 5-HT1B levels are associated with reduced anxiety in animals that have not been exposed to stress. PMID: 12957218
  30. activation of ventral tegmental area 5-HT1B receptors increases mesolimbic dopamine neuron activities PMID: 15328035
  31. These findings strongly suggest that the specific anti-aggressive effects of 5-HT(1A) and 5-HT(1B) receptor agonists are predominantly based on reduction rather than enhancement of 5-HT neurotransmission during the combative social interaction. PMID: 16310183
  32. Collectively these findings demonstrate that 5-HT1B receptor function is persistently altered by cocaine self-administration. PMID: 17074068
  33. Increased 5-HT(1B) receptor activation in NAcc shell projection neurons intensifies both the rewarding and negative properties of cocaine use. PMID: 17509084
  34. Subarachanoid hemorrhage up-regulates 5-HT1B receptors. Changes parallel successive decrease in cerebrovascular blood flow. PMID: 17873013
  35. The rates of development and the segregation of thalamo-cortical fibers were normal in nutritionally restricted rats. SERT, but not 5-HT(1B) immunoreactivity, was decreased in the primary somatosensory cortex during barrel specification. PMID: 18207350
  36. The HA1B/GFP vector reveals a useful tool to induce a targeted increase of 5-HT(1B) heteroreceptors on cholinergic neurons in selected CNS regions, which provides interesting perspectives for functional approaches at more integrated levels. PMID: 18502320
  37. These data suggest that long-term changes in 5-HT(1B) receptor function occur after serotonergic damage caused by a single dose of MDMA. PMID: 18638459
  38. Data show that middle cerebral artery occlusion results in upregulation of cerebrovascular 5-hydroxytryptamine 5-HT1B receptors. PMID: 18793415
  39. The 5-HT(1B) receptors were significantly greater in the FSL rats than in either the SPD or FRL rats. In addition, the FRL rats have 5-HT(1B) receptor densities significantly lower in many brain regions than the SPD rats. PMID: 19121358
  40. Cerebral arteriole smooth muscles possess 5-HT1a, 1b, 2 (G-protein-coupled type), and 3 (ion channel type) receptors. PMID: 19556691

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: rno:25075

STRING: 10116.ENSRNOP00000017411

UniGene: Rn.138109

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