Recombinant Rat Lysophosphatidylcholine acyltransferase 2(Lpcat2)

Code CSB-CF013053RA
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names Lpcat2
Uniprot No. P0C1Q3
Alternative Names Lpcat2; Aytl1; Aytl1a; Lpcat2a; Lysophosphatidylcholine acyltransferase 2; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; EC 2.3.1.23; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-AGP acyltransferase 11; 1-AGPAT 11; EC 2.3.1.51; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; EC 2.3.1.67; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 1
Species Rattus norvegicus (Rat)
Expression Region 1-544
Target Protein Sequence MNRCAEAAAVAATVPGSGVGDSGLRPPMVPRQASFFPPPVPNPFVQQTRISAARRLQMILLGIILLPVRALLVGLVLLLAWPFAVISTVCCPKKLTHPISDWRRKITQPALKFLGRAMFFSMGFRVTVKGKVASPLEAPIFVVAPHSTFFDGIACVVAGLPSLVSRNENAQTPLVGRLLRALQPVLVSRVDPDSRKNTINEIKKRAMSGGEWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCVLTFCQLFTKVEVEFMPVQAPSEEERNDPVLFASRVRNLMAEALEIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKLDWDGIRKHLDEYASIASSAKGGRIGASSAKGGRIGPVSDVLRQLFALFDRNNDGSIDFREYVIGLAVLCNPANTEDIIQVAFKLFDVDEDGYITEEEFCTILQASLGVPDLNVSGLFREIAQGDSVSYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPEDVQTAPSVASNKVSPESHEEGTSGKKVD
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Also catalyzes the conversion of 1-acyl-sn-glycero-3-phosphocholine to 1,2-diacyl-sn-glycero-3-phosphocholine. Involved in the regulation of lipid droplet number and size.
Subcellular Location Endoplasmic reticulum membrane, Single-pass type II membrane protein, Golgi apparatus membrane, Single-pass type II membrane protein, Lipid droplet
Protein Families 1-acyl-sn-glycerol-3-phosphate acyltransferase family
Database Links

STRING: 10116.ENSRNOP00000022359

UniGene: Rn.81758

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