Recombinant Rat Melanopsin (Opn4)-VLPs

Code CSB-MP851271RA
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Product Details

Target Names
Uniprot No.
Research Area
Neuroscience
Alternative Names
(Opsin-4)
Species
Rattus norvegicus (Rat)
Source
Mammalian cell
Expression Region
1-474aa
Target Protein Sequence
MNSPSESRVPSSLTQDPSFTASPALLQGIWNSTQNISVRVQLLSVSPTTPGLQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCRNRGLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGETGCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGWSAYVPEGLLTSCSWDYVTFTPLVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGRACEGCGESPLRRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVGFAGYSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYRAAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGQKRQESLGSESEVGWTDTETTAAWGAAQQASGQSFCSHDLEDGEVKAPSSPQEQKSKTPKTKRHLPSLDRRM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it's possible to develop.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from PBS, 6% Trehalose, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Photoreceptor that binds cis-retinaldehydes. Contributes to pupillar reflex, photoentrainment and other non-image forming responses to light. May be involved in the optokinetic visual tracking response. May be involved in the regulation of retinal hyaloid vessel growth and regression.
Gene References into Functions
  1. during retinal degeneration, the expression of melanopsin was significantly decreased, while vector-mediated overexpression of melanopsin delayed the loss of visual function in rats. PMID: 26572076
  2. Following ON injury or retrograde tracing there is a transient melanopsin downregulation that should be taken into account when assessing m(+)RGC survival. PMID: 26176868
  3. Data indicate that the phosphorylation status of melanopsin influences its functions. PMID: 25378407
  4. Early light exposure can increase the number of melanopsin immunopositive retinal ganalion cells through a process dependent on intrinsic photosensitive spiking activity. PMID: 23541830
  5. We found that light and darkness are important for regulation of melanopsin protein expression whereas input from a retinal networks regulates melanopsin mRNA expression. PMID: 23187103
  6. overexpression of mir133b decreased TH and D2 receptor expression as well as dopamine production, which finally resulted in reduced melanopsin expression PMID: 22101014
  7. Melanopsin expression defines a subset of RGCs that play a broad role in regulation of nonvisual photoreception, providing projections that contribute to circadian entrainment, negative masking, regulation of sleep-wake states, and the pupillary reflex PMID: 12904470
  8. Melanopsin, in itself, is not necessary for circadian photoreception. In fact, it appears that of the known photoreceptor systems, none, in and of itself, is necessary for circadian photoreception. PMID: 14500998
  9. During rat retinal development melanopsin is expressed at prenatal day 18 in cells of the inner neuroblast layer. and During early postnatal development a melanopsin immunoreactive dendritic network is formed in the inner plexiform layer. PMID: 15073514
  10. We investigated the effect of photoreceptor degeneration on melanopsin mRNA regulation in RCS/N-rdy rats (Royal College of Surgeons rats with a defect in the retinal dystrophy gene). PMID: 15509757
  11. Data suggest that retinal ganglion cells adapt their expression of the putative circadian photopigment melanopsin to environmental light and darkness. PMID: 16186625
  12. Regulation of melanopsin expression [review] PMID: 16687290
  13. melanopsin-containing RGCs have synaptic contact with PKC/CtBP2-containing rod bipolar cells PMID: 17652756
  14. the daily and photoperiod-dependent regulation of the melanopsin gene does not rely on a circadian oscillator but is directly illumination-dependent PMID: 18001324
  15. The PI3 K/Akt pathway was found to play a role in maintaining the survival of melanopsin-expressing retinal ganglion cells after injury. PMID: 18512147

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Perikaryon.
Protein Families
G-protein coupled receptor 1 family, Opsin subfamily
Tissue Specificity
Eye; expressed in a photosensitive subset of retinal ganglion cells (at protein level).
Database Links
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