Recombinant Rat Platelet-activating factor receptor (Ptafr)

Code CSB-CF018946RA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Ptafr
Uniprot No.
Alternative Names
Ptafr; Platelet-activating factor receptor; PAF-R; PAFr
Species
Rattus norvegicus (Rat)
Expression Region
1-341
Target Protein Sequence
MEQNGSFRVDSEFRYTLFPIVYSVIFVLGVVANGYVLWVFATLYPSKKLNEIKIFMVNLT VADLLFLMTLPLWIVYYSNEGDWIVHKFLCNLAGCLFFINTYCSVAFLGVITYNRYQAVA YPIKTAQATTRKRGITLSLVIWISIAATASYFLATDSTNVVPKKDGSGNITRCFEHYEPY SVPILVVHIFITSCFFLVFFLIFYCNMVIIHTLLTRPVRQQRKPEVKRRALWMVCTVLAV FVICFVPHHVVQLPWTLAELGYQTNFHQAINDAHQITLCLLSTNCVLDPVIYCFLTKKFR KHLSEKFYSMRSSRKCSRATSDTCTEVMMPANQTPVLPLKN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.
Gene References into Functions
  1. In hypoxic tissue, PAFR protein expression is up-regulated, but is reduced in the bilobalide treated tissue. PMID: 21391918
  2. These data show the histological localization of PAF synthases and its receptor in the spinal cord following peripheral nerve injury. PMID: 22296727
  3. PAF inhibition interkinetic nuclear migration (IKNM) in retinal proliferating progenitors depended on the PAF receptor, Erk and p38 pathways and Chk1 PMID: 21298035
  4. differential activation of both G(i) and G(q) by PAFR likely mediate specific as well as redundant signaling pathways PMID: 16920964
  5. PAF receptor gene expression may be subject to down-regulation in the perifocal regions of cerebral infarction after cerebral ischemia-reperfusion. PMID: 17268849
  6. Platelet activating factor receptor (PAF-R) plays an important role in occurrence and development of severe acute pancreatitis. BN52021 exerts biological effects through competitively inhibiting the binding of increased both PAF and PAF-R expression. PMID: 17589953
  7. Cirrhosis sensitizes hepatic stellate cells to PAF by elevating its receptor level PMID: 18186558

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Present in almost all organs including spleen, small intestine, kidney, lung, liver and brain.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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