Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

S1pr2

Uniprot No.

P47752

Research Area

Epigenetics and Nuclear Signaling

Species

Rattus norvegicus (Rat)

Source

in vitro E.coli expression system

Expression Region

1-352aa

Target Protein Sequence

MGGLYSEYLNPEKVQEHYNYTKETLDMQETPSRKVASAFIIILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGPVTLSLTPLQWFAREGSAFITLSASVFSLLAIAIERQVAIAKVKLYGSDKSCRMLMLIGASWLISLILGGLPILGWNCLDHLEACSTVLPLYAKHYVLCVVTIFSVILLAIVALYVRIYFVVRSSHADVAGPQTLALLKTVTIVLGVFIICWLPAFSILLLDSTCPVRACPVLYKAHYFFAFATLNSLLNPVIYTWRSRDLRREVLRPLLCWRQGKGATGRRGGNPGHRLLPLRSSSSLERGLHMPTSPTFLEGNTVV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

41.6 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In the in vitro E. coli expression system, the recombinant rat sphingosine 1-phosphate receptor 2 (S1pr2) is produced through the gene fragment that encodes the full-length rat S1pr2 protein (1-352aa). The process involves cloning the S1pr2 gene fragment along with a C-terminal 10xHis-tag into a suitable expression vector with the necessary regulatory elements. The recombinant plasmid DNA is then isolated and purified. In vitro transcription is performed using the purified plasmid DNA to generate mRNA, which serves as a template in the subsequent in vitro translation reaction. The translation reaction is carried out using an E. coli cell-free extract supplemented with energy sources and amino acids. After incubation, the recombinant S1pr2 protein is purified from the cell culture medium. The purified protein exhibits a purity level of up to 85% as confirmed by SDS-PAGE analysis. On the gel, the recombinant S1pr2 protein appears as a distinct band with a molecular weight of approximately 35 kDa.

Target Background

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues. Receptor for the chemokine-like protein FAM19A5. Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration.

Gene References into Functions

Sphingosine-1 phosphate (S1P)-mediated activation of S1PR2 plays an important role in regulating intestinal epithelial cell proliferation and migration. S1P significantly upregulated the expression of c-Myc, cyclin D1, E-cadherin and zona occluden-1 (ZO-1), which was completely inhibited by downregulation of S1PR2 expression with a shRNA. PMID: 27814635
TGR5 activation reduced ICAM-1, TGF-beta1 and FN expressions induced by high glucose in glomerular mesangial cells, the mechanism might be through suppressing S1P/S1P2 signaling, thus ameliorating diabetic nephropathy. PMID: 27317945
S1P contracted control colon via S1PR1 and S1PR2 and inflamed colon exclusively via S1PR2. Calcium influx (control) or release (inflamed) and calcium sensitization are involved in S1P-induced contraction. Exacerbated response to S1P in colitic colon segments may explain altered colonic motility reported in patients and experimental models of inflammatory bowel disease. PMID: 28493876
Inhibition of S1PR2 down-regulate the expressions of SphK1 and MCP-1 in glomerular mesangial cells under high glucose. PMID: 25575056
Conjugated bile acids promote cholangiocarcinoma growth through S1PR2. PMID: 24700501
extracellular S1P induces COX-2 expression via activation of S1P2 and subsequent Gi and p42/p44 MAPK-dependent signaling in renal mesangial cells leading to enhanced PGE2 formation and cell migration that essentially requires COX-2. PMID: 24064301
S1P may play an important role in the pathophysiology of portal hypertension with Rho kinase activation by way of S1P(2) . PMID: 22505286
Results indicate that the impaired functions (such as chemotactic, wound healing, and morphogenetic responses) in senescent endothelial cells are mediated by the S1P(2) receptor. PMID: 22139303
S1P2 receptor was significantly up-regulated under diabetic condition. S1P2 receptor mediated fibronectin expression through the activation of S1P-S1P2-MAPK axis in mesangial cells under high glucose condition. PMID: 22406263
Conjugated bile acids activate the ERK1/2 and AKT signaling pathways primarily through S1P(2) in primary rodent hepatocytes. PMID: 21932398
S1P modulates central intracellular signaling cascades and is antiproliferative in PC Cl(3) cells. S1P(2) receptor expression is modulated by insulin and TSH, two central growth factors in thyroid cell regulation. PMID: 15713536
S1P (sphingosine-1-phosphate receptor)1 and S1P3 receptors promote, whereas S1P2 receptors antagonize, smooth muscle cell proliferation and phenotypic modulation in vitro in response to sphingosine-1-phosphate (S1P), or in vivo after vascular injury PMID: 18535287
Data demonstrate the importance of S1P and the S1P(2) receptor as regulators of allergen-induced activation of mast cells in their natural environment in the rat lung. PMID: 19636535

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Expressed in all developing tissues with highest levels detected in primitive, transformed cells. Relative abundance: lung > kidney = skin = gut > spleen > brain > liver.

Database Links

KEGG: rno:29415

STRING: 10116.ENSRNOP00000028034

UniGene: Rn.2491

Code	CSB-CF020651RA
Abbreviation	Recombinant Rat S1pr2 protein
MSDS	MSDS Datasheet
Size	$1040
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2)

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