Recombinant Rat Synaptotagmin-4 (Syt4)

Code CSB-CF023040RA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Syt4
Uniprot No.
Alternative Names
Syt4; Synaptotagmin-4; Synaptotagmin IV; SytIV
Species
Rattus norvegicus (Rat)
Expression Region
1-425
Target Protein Sequence
MAPITTSRVEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRRSAKSNKTPPYKFVHVLKGVDIYPENLSSKKKFGGDDKSEAKRKAALPNLSLHLDLEKRDLNGNFPKTNPKAGSSSDLENVTPKLFPETEKEAVSPESLKSSTSLTSEEKQEKLGTLFLSLEYNFEKKAFVVNIKEAQGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPVFDETFTFYGVPYPHIQELSLHFTVLSFDRFSRDDVIGEVLVPLSGIELSDGKMLMTREIIKRNAKKSSGRGELLVSLCYQSTTNTLTVVVLKARHLPKSDVSGLSDPYVKVNLYHAKKRISKKKTHVKKCTPNAVFNELFVFDIPCESLEEISVEFLVLDSERGSRNEVIGRLVLGATAEGSGGGHWKEICDFPRRQIAKWHMLCDG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Synaptotagmin family member which does not bind Ca(2+). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses. Plays a role in dendrite formation by melanocytes.
Gene References into Functions
  1. neuronal activity increased dense core vesicle capture via JNK-dependent phosphorylation of the S135 site of Synaptotagmin-4 PMID: 29166604
  2. The increased expression of Synaptotagmin IV in optic nerve crush eyes displays the possible neuroprotective effect of bone marrow mononuclear cell treatment PMID: 22695963
  3. Immunocytochemistry in rat chromaffin cells shows differential distribution of synaptotagmin-1, -4, -7, and -9. PMID: 21287204
  4. These data support an inhibitory effect of syt IV on release of vesicles and their transmitter content PMID: 20735850
  5. Rat and Drosophila synaptotagmin 4 have opposite effects during SNARE-catalyzed membrane fusion. PMID: 20688915
  6. The effects of syt IV on fusion pores are discussed in terms of structural models and kinetic mechanisms. PMID: 20303854
  7. present several lines of evidence showing that the Syt IV is normally localized in the Golgi or immature vesicles at the cell periphery and is sorted to fusion-competent mature dense-core vesicles in response to short nerve growth factor stimulation PMID: 12446703
  8. NMR analysis of Ca2+ binding to synaptotagmin 4 PMID: 15311271
  9. synaptotagmin IV protein trafficking from the Golgi to the cell periphery is regulated by forskolin PMID: 15496596
  10. Cells with overexpressed rat SytIV were unaltered in basal release probability, Ca2+ dependence of release, short-term plasticity, & fusion pore kinetics. Mutant Syt I (less Ca2+ affinity) reduced release probability & altered Ca2+ dependence of release. PMID: 16407532
  11. Downregulation of Syt IV reduced Ca(2+)-triggered exocytosis of dense core vesicles. Syt I silencing, however, had no effect on vesicular release. PMID: 18468511
  12. Given the neuroendocrine functions of the posterior pituitary, changes in Syt IV levels could be involved in endocrine transitions involving alterations in the release of the neuropeptides oxytocin and vasopressin PMID: 19136969
  13. Expression of Syt4 increases 9-fold at night in the rat pineal gland; treatment with dibutyryl cyclic AMP increases expresssion. PMID: 19103603

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Subcellular Location
Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle membrane; Single-pass membrane protein.
Protein Families
Synaptotagmin family
Tissue Specificity
Widely expressed. Expressed in the brain. Expressed in pituitary gland, cerebellum, cortex, hypothalamus and hippocampus.
Database Links
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