Recombinant Saccharomyces cerevisiae GPI-anchor transamidase(GPI8)

Code CSB-CF017974SVG
Product Type Transmembrane Protein
Size Pls inquiry other sizes
Uniprot No. P49018
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Sequence NNTDAAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSMYRTVKRLGIPDSQIILMLSDDVACNSRNLFPGSVFNNKDHAIDLYGDSVEVDYRGYEVTVENFIRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQMYEKKRYNEIFFMIDTCQANTMYSKFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDRFTYYCLDFLEQIDKNSTLTLQDLFDSFTFEKIHSHVGVRTDLFDRNPSEVLITDFFANVQNVIPDDSKPLSVSHYHHYKDHIDTAQYELNNNVLDLALETYRKNNQSSKIEKKIKDIKSTSVLDVDIDSNECFFTSFKQSATIILALIVTILWFMLRGNTAKATYDLYTN
Source in vitro E.coli expression system
Gene Names GPI8
Protein Names Recommended name: GPI-anchor transamidase Short name= GPI transamidase EC= 3.-.-.-
Expression Region 23-411
Tag Info N-terminal 10xHis-tagged
Protein Description Full Length of Mature Protein
Storage Store at -20°C, for extended storage, conserve at -20°C or -80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.
Subcellular Location Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families Peptidase C13 family
Database Links

KEGG: sce:YDR331W

STRING: 4932.YDR331W

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