Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane magnesium transporter MFM1 (MFM1)

Code CSB-CF311623SVG
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
MFM1
Uniprot No.
Alternative Names
MFM1; LPE10; YPL060W; Mitochondrial inner membrane magnesium transporter MFM1; MRS2 function modulating factor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
36-413
Target Protein Sequence
FSRQMPKVDPDNTAAMLLQKNLIQRNNMLYGYGSGTIRCTLLDSTGRAKSPLVEIKREDL VSKHGLLPRDLRKIEKSRKNDLVPSLLVRENSILISLLTVKALIKPDMVIIFDSAGSGIT LNSEAHKDFINDMKLRLKNQETSELNSDPLPYEFRALETIFISALSNLTSEMKVLLTICK GVLQDLEFSITRDKLRFLLGQNKKLSSFNKKAVLVKDMLDDLLEQDDMLCDMYLTDKKAG KIRVQDDHTEIEMLLETYHNYVDEIVQKSESAISDVKTTEEIINIILDSNRNELMLLGIR YAIGMLSLGGALFLGSIYGMNLESFIEESNYAYLTVTILGLISTVWLYAKGIRHLHKLQR MTLLSKIKTDSVHELLKK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mitochondrial inner membrane magnesium transporter required for mitochondrial magnesium homeostasis. Modulates the conductance of the MRS2 channel. Involved in the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing.
Gene References into Functions
  1. The interplay of Lpe10p is of central significance for the transport of Mg(2+) into mitochondria of S. cerevisiae. PMID: 20653776
Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families
CorA metal ion transporter (MIT) (TC 1.A.35) family
Database Links

KEGG: sce:YPL060W

STRING: 4932.YPL060W

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